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Retroviral Delivery and Anti-HIV Testing of Hammerhead Ribozymes

 Last Update: 2020-11-23
 Source: Internet
 Author: User

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Successful ribozyme-mediated gene therapy for HIV infection has to take into account several factors:

1.	The therapeutic gene must interfere with or shut down the expression of essential viral genes.
2.	This requires a vector system for gene delivery and expression in HIV-sensitive cells.
3.	To bypass the high genetic variability of the HIV-1 replication process, the target sequence should be chosen in highly conserved sequences of HIV-1. These fortunately are mostly present in the essential regulatory genes (tat, rev ) or sequences (PBS, U5), which are required to produce infectious viral particles (1 ).

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