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RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer

 Last Update: 2020-11-29
 Source: Internet
 Author: User

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dna rna hybrid

synthetic

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The RNA electrophoretic mobility shift assay is a simple and rapid method for visualizing the existence of specific RNA-protein interaction. We have developed a useful method for the detection of mRNA-binding proteins using fluorescence-labeled synthetic RNA. In this method, RNA was prepared by in vitro transcription using Texas Red-labeled nucleotides. This method has higher resolution than the conventional method using slab gels, is safer, and provides results rapidly because of the use of fluorescence detection.

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