Salmonella infects host cell schematics, the active selection mechanism of histaline kinase EnvZ enzyme regulated by pH in salmonella.

The two-component signal transduction system (TCS) is the most important signal transduction system in the bacteria body, regulating most of the life activity of bacteria.
as a potential target for new antimicrobial drugs, bacteria TCS has long been a hot topic in related fields.
TCS consists of histtidine kinase (HK) and reaction-regulating protein (Response Regulator, RR).
like relay inglisile, relaying batons between the team members, the transduction of signals between them through the transmission of phosphoric acid groups, and the phosphorylation-adjusted protein regulates the expression of some special genes.
THE HK protein is a sensor for bacteria that responds by sensing different environmental conditions.
the presence of dozens of pairs of TCS in most bacteria, they regulate the vast majority of bacterial physiological processes, including bacterial chemometricity, perceived osmosis pressure, spore formation, metabolism of nutrients, and biosynthesis of secondary metabolites.
, for example, the genome of E. coli encodes 62 TCS proteins and participates in the regulation of drug-resistant, metabolic and transshipment physiological processes.
previous studies have found that salmonella in rat typhoid invades host cells by swallowing cells.
salmonella two-component system plays an important role in the infection and release of salmonella by sensing changes in the pH of the environment.
in this process, histaine kinase in the two-component system can switch between different functions, but its molecular mechanism is not clear.
recently, Jiang Ling of the Wuhan Institute of Physics and Mathematics of the Chinese Academy of Sciences successfully captured the protein transient complex in the process of phosphoric acid transmission in cooperation with Duke University of the United States and the Wuhan Virus Research Institute of the Chinese Academy of Sciences.
researchers found that the dual-function protein HK853 enzyme is actively regulated by pH, and that HK853 will change in conformationolire in acidic environment, which reduces phosphase activity, and found that pH changes regulate the expression of toxic factors through intracellular and extracellular experiments of EnvZ-OmpR, a two-component system of salmonella, which affects the ability of bacteria to infect.
based on this, the researchers proposed the enzyme-stimulating mechanism in which HisKA family kinase exercised phosphatase activity.
Since the two-component signaltransmission system is currently found only in bacteria, paleobacteria and plants, but not found in humans and other mammals, the two-component transduction system can be designed according to the mechanism, the two-component transduction system as a drug target, killing only bacteria and harmless to humans.
the results of this study are of reference value to understanding the transduction mechanism of bacterial signals, the toxic secretion mechanism of acid-resistant pathogenic bacteria such as salmonella, and the research and development of new antimicrobial drugs.
research in the journal Nature-Communications, based on the research of a pH-gated conformational switch regulations the phosphatase activity of the bifunctional HisKA-family histidine kinases.
the research was supported by the Ministry of Science and Technology, the National Natural Science Foundation of China, the National Institute of Medical Sciences, etc.
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