Science Sub-Journal: Developed a two-color RT-LAMP test to detect RNA from new coronavirus viruses.

The new coronavirus SARS-CoV-2, which causes coronavirus disease (COVID-19) in 2019, is now raging around the world.
this, humanity faces a major public health challenge.
is critical to rapidly detect existing SARS-CoV-2 infections and assess virus transmission.
the method of detecting viral RNA based on reverse transcription loop-mediated isothermal amplification, RT-LAMP has the potential to become a simple, scalable and widely applicable method of detection.
RT-LAMP detection requires incubating at constant temperatures compared to methods based on reverse transcription quantitative polymerase chain reaction (RT-qPCR), so there is no need for complex instrumentation.
in a new study, researchers from the German Center for Infection Research, the German Cancer Research Center and the University of Heidelberg tested a two-color RT-LAMP test for SARS-CoV-2 virus RNA using a set of citations for the SARS-CoV-2 N gene.
recently published in the journal Science Translational Medicine, the paper is titled "A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples".
images from Science Translational Medicine, 2020, doi:10.1126/scitranslmed.abc7075.
the authors obtained excess RNA samples from 768 pharynx swab samples from individuals undergoing COVID-19 testing and RT-LAMP tests on those samples.
they measured the sensitivity and specificity of using RT-LAMP to detect SARS-CoV-2 virus RNA.
compared to RT-qPCR detection methods using a set of highly sensitive citants, the authors found that RT-LAMP detection methods reliably detected SARS-CoV-2 RNA with a cycle threshold (Ct) of no more than 30 during RT-qPCR tests with a sensitivity of 97.5% and a specificity of 99.7%.
the authors also developed a direct RT-LAMP (swab-to-RT-LAMP) testing method for pharynx swabs that do not require prior RNA separation steps, which retains excellent specificity (99.5%), but is less sensitive than RT-LAMP testing (86% sensitivity for CT-lt;30 swab samples during RT-qPCR testing).
addition, the authors developed a multi-sequencing method (LAMP-sequencing, LAMP sequencing) as a diagnostic validation procedure to detect and document the results of RT-LAMP reactions.
: Viet Loan Dao Thi et al. A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples. Science Translational Medicine, 2020, doi:10.1126/scitranslmed.abc7075. This article is sourced from Bio Valley, for more information please download Bio Valley APP (