Scientists have developed unprecedented tools to visualize DNA repair

The human body is composed of trillions of cells, and each cell suffers more than 10,000 DNA damages every day

Martinez explained that once DNA damage occurs, such as a DNA double-strand break, the cell activates a mechanism called DNA damage response, just like "calling emergency services

The purpose of chemotherapy is to kill tumor cells by inducing DNA damage, leading to the collapse and death of cancer cells

Researchers have developed a new method that can analyze this process with unprecedented detail and precision with the help of the machine learning analysis method designed by the CNIO confocal unit

Researchers used high-throughput microscopy technology to obtain thousands of cell photos after inducing genetic damage

But the authors decided to go a step further and visually inspect these 300 proteins after gene damage

"We found that many proteins will adhere to damaged DNA, while others will be the opposite: they will stay away from damaged DNA

One of the proteins discovered is PHF20

These technologies provide new opportunities for studying DNA repair and manipulation of DNA

This work was funded by the Spanish Ministry of Science and Innovation, Carlos III Institute of Health, National Institutes of Health, Marie Curie Joint Fund FP7, Massachusetts General Hospital, European Research Council, and Canadian Natural Science and Engineering Research Council.

Article title

Assessing kinetics and recruitment of DNA repair factors using high content screens