Serological diagnosis of Helicobacter pylori infection in children

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Serum
diagnosis of helicobacteria infection in childrenHelicobacterrobacteria (Hp) is closely related to gastrointestinal disease in children. At present, the conventional methods used to detect HP infection are bacteria
culment
, biopsy mucous membrane direct smear grading grading, urea enzyme decomposition test, pathology
tissue
slibe
and other methods. These examinations are cumbersome and time-taking, require gastroscopy and mucous tissue, which is not easy for children to accept.
14
The C urea breath test is simpler, but radioactive and not harmful to children. In this paper, serological methods are used to detect HP infection in children, and the effect is good. 1 objects and methods72 cases of gastrointestinal diseases, aged 4 to 13. 48 cases for men and 24 cases for women. Among them, 36 cases of repeated abdominal pain or umbilical pain for more than 1 month, 12 cases of anorexia and indigestion, and 24 cases of chronic diarrhea and malnutrition. Tuberculosis, hepatitis, intestinal parasitic disease and abdominal epilepsy were excluded from all cases. The control group had 60 children of normal age, of whom 39 were men and 21 were women. All of the blood taken from the outer perivenous veins were 1 ml to 2 ml, separated hemorrhagic clear, stored in the refrigerator at -20 degrees C to be tested hp
antibody
. With hp
antigen
is a 40-well polystyrene reaction plate, which is operated by conventional indirect enzyme-linked immunosorption method (ELISA). The serum was diluted to 1:200, the spicy root peroxidase-labeled sheep anti-human IgG, the substrate phthalates, with sulfuric acid to terminate the reaction, at 490nm to read the light density value (OD). Each serum to be tested is porous, and each plate has a positive, negative and blank control. Results The ratio of the serum OD value (P) to the negative control OD value (N) was used to indicate that P/N≥2 was positive and <2 was negative.2 Results 72 cases of serum HP antibody IgG in children with chronic gastrointestinal diseases, 54 were positive, 18 were negative, negative rate was 75%, 60 normal children of the same age, 18 were positive, 42 negative, positive rate was 30%. By
statistical
X
2
test, P<0.01, between the two groups has significance.3 theory the routine diagnosis of HP infection through gastroscopy to obtain gastric mucous membrane specimens, difficult to accept in children. Since HP stimulates the body to produce specific antibodies, IgG, Loffeld, etc., believes that serological examination can replace endoscopy to diagnose Hp infection. Pan Zhijun and other reports, in adults with ELISA method detection and tissue culture, smear detection of HP infection results compared to the later standard, ELISA method sensitivity of 95%, specificity of 90%. We tested serological HP antibody IgG on 72 children with recurrent abdominal pain, anorexia and chronic diarrhea, 54 of whom were positive (75%). This suggests that Hp infection is an important cause of chronic gastrointestinal disease in children. Of the 60 children of the same age, 18 were HP antibody IgG-positive (30%). There are no symptoms of the digestive tract, and they may have had an HP infection, but the body has cleared hp. Antibodies still exist because a significant number of normal people can automatically clear hp infections.serological diagnosis of HP infection, non-traumatic, simple, especially suitable for children and hp infection screening. This method is not affected by the recent use
antibiotics
, can be repeated testing, dynamic observation, evaluation of efficacy. If the HP infection has been cleared, the serum HP antibody titration gradually decreases and eventually disappears.Hu Boyuan Yu Wenxin Yu LihuaNanjing Children's Hospital (Nanjing 210008)