Several common microbial basic experiments
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Last Update: 2021-01-21
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Source: Internet
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Author: User
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this paper introduces the purpose, principle and content of several common
microorganisms
basic tests, so that comrades who have just come into contact with microorganisms have a basic understanding of the experiment.
Experiment I Commonly used
culture
-based preparation, sterilization and disinfection
First, the experimental purpose 1, master the preparation of
fleat
general methods and procedures; master dry hot bacteria, autobaric steam sterilization and
filtration
sterilization operation methods;
2, understand the preparation principle of the culture base, understand the common sterilization, detoxification basic principles and methods.
, the principle of experimentA culture base is a mixture of nutrients artificially made in proportion for microbial growth, reproduction and anabolic products. The raw materials of the culture base can be divided into carbon source, nitrogen source, inorganic salt, growth factor and water. Depending on the type of microorganism and the purpose of the experiment, the culture base also has different kinds and preparation methods.
beef paste proteinis one of the most widely used and common bacterial base media, sometimes referred to as ordinary media. Because this medium contains the most basic nutrients needed for normal cell growth and reproduction, it can be used for microbial growth and reproduction.
, high-pressure steam sterilization method is mainly through heating to denature
protein
to achieve the effect of killing microorganisms.
, reagents and equipment1, equipment test tube, triangular bottle, beech, gauge, glass rod, medium, sub-packer,
balance
, horn spoon, high-pressure steam sterilization pot, pH paper, cotton, verse paper, marker pen, hemp rope, gauze, straw, petri dish, electric oven, syringe, micropore membrane filter, tweezers, etc.
2, reagent beef paste, protein , NaCl,
agar
4, experimental content 1, weighing →solvation→ tune pH→filtration→ sub-pack→ Garces → →sterilization→sterile inspection
2, dry heat sterilization: loaded into the sterilization items →healthing→
thermostat
shaker→ cooling→ out of the box
3, high-pressure steam sterilization: water →charged items→charged→ heated→ drained cold air →pressurized→ constant pressure→ buck back to zero→→ →Sterile inspection
4, filtration sterilization: assembly sterilization→ connecting→pressurization →sterile filtration→ cleaning sterilization
5, key steps and precautions1, to be strictly formulated according to the formula.
2, tune pH don't overdring.
3, dry heat sterilization should pay attention to items do not stack too tight, pay attention to the temperature of the time control, 70oC below the discharge, take.
4, autobaric sterilization should pay attention to the items do not too much, after heating to exclude cold air, when buck back to zero.
5, filtration sterilization should pay attention to the sterilization of various components, pressure should be appropriate, not too fast, membrane should pay attention to cleaning preservation.
, thinking about the1, after the culture base is good, why must be sterilized immediately? How do I check that the culture base after sterilization is sterile?
2, what problems should be paid attention to during the operation of the preparation of the culture base? Why?
conditions should be met for the culture of cultured microorganisms? Why?
the preparation principles of the culture base in The 4th and 4th?
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