 Home > Biochemistry News > Biotechnology News > Site-Specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells

Site-Specific Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells

 Last Update: 2020-12-25
 Source: Internet
 Author: User

Tags

generic amino acid

amino acid peptide

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

Expanding the repertoire of genetically encoded amino acids in cultured mammalian cells requires the expression of the bacterial or archaeal pair of a tRNA and an aminoacyl-tRNA synthetase variant engineered to be specific to the amino acid, along with the supplementation of an unnatural amino acid in the growth medium. The expression of the pair is generally achieved by transfecting the cultured cells with the plasmids bearing the genes encoding the exogenous pair of translation molecules. Here, we provide a description of some of these plasmids and protocols for transfecting cells with the plasmids and preparing growth media supplemented with unnatural amino acids, to facilitate their incorporation into proteins at specific sites.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

L-Ascorbate-2-Monophosphate Feed Grade

CAS No.: 109620-90-8

Feed Grade

Potassium Sorbate from reliable manufacturer

CAS No.: 590-00-1

Food Grade

Doxycycline Hydrochloride

CAS No.: 10592-13-9

Pharmaceutical Grade

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.