Strategies and effect molecules for the defense and removal of in-cell pathogens in vertebrates.

Recently, the Journal of Infectious Diseases of the American Society of Infectious Diseases published online the latest research results of Zhang Yun's team at the Kunming Institute of Zoology of the Chinese Academy of Sciences.
the study reveals strategies and effect molecules for vertebrate defense and removal of in-cell pathogens, which are of practical significance for the study of new disease treatment strategies and drugs.
pathogens infected in in-cell bacteria, viruses, etc. use a variety of strategies to adapt and change the in-cell environment of the host cell, escaping the host's immune defenses.
in this process, many intracular pathogens use the cell physiological endocellular vesicle pathway to infect cells, and adapt to the acidized environment of intra-cell swallowing vesicles to achieve the purpose of infection.
Exogenic chemicals such as ammonium chloride and chloroquine can inhibit infection of certain viruses and intracular parasites by altering the acidification environment in which vesicles are swallowed in cells, but whether host cells have corresponding defense strategies, the identity of endogenic effect molecular machines, and regulatory networks have been important issues that researchers have been trying to explore.
The author and researcher Zhang Yun led the team to discover the vertebrate's first new aperture-forming protein and clerical factor complex betagamma-CAT from the amphibian's big bell bell, and found that the endogenous protein machine has the characteristics of stimulating cell vesicle formation, forming membrane channels and activating inflammatory bodies in the in-cell swallow/lysome pathway.
on this basis, the first author of the article, Kunming Institute of Zoology Associate Researcher Li Sheng'an and doctoral student Liu Long and other researchers, further revealed that the complex has the function of regulating the intracular vesicle acidification environment.
In the host immune defense, studies modeled on in-cell pathogen mononucleosis listeria have shown that the protein machine can limit listeria's escape from in-cell vesicles, and at the same time stimulate extracellular excretion to expel in-cell infection pathogens, effectively resisting fatal Listeria infections.
the research was funded by the National Natural Science Foundation of China, the Yunnan Joint Fund, and the Yunling Scholars.
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