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Structural Genomics of Minimal Organisms: Pipeline and Results

 Last Update: 2021-03-03
 Source: Internet
 Author: User

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The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens
Mycoplasma genitalium
and
M. pneumoniae
. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93% of all soluble proteins of
M. genitalium
is avail able. This chapter summarizes the approaches taken by the authors' center.

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