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Peptide nucleic acids (PNAs) have very attractive properties for applications in nuclear medicine. Because PNAs have highselectivity for
DNA
/RNA recognition, resistance to nuclease/protease degradation, and high thermal and radiolytic stabilities,PNA bioconjugates could transform the areas of diagnostic and therapeutic nuclear medicine. In this book chapter, we reporton the current developments towards the preparation of radiometal-containing PNA constructs and summarize the protocols forlabeling these probes with
99m
Tc,
111
In,
64
Cu,
90
Y, and
177
Lu.