Synthesis of conjugated linoleic acid

Introduction: conjugated linoleic acid can reduce the content of blood lipid, enhance the immune function of human body, especially for the content of triglyceride and cholesterol in human body, so what are the synthesis methods of conjugated linoleic acid? Let's take a look at it together with the editor Conjugated linoleic acid can decompose the excess fat in human body, supplement the energy of muscle, promote the combustion of fat, enhance muscle strength, and even improve the content of lean muscle So what are the synthesis methods of conjugated linoleic acid? Let's learn about it with the safety net (1) Under the condition of natural vegetable oil as raw material, KOH as catalyst and propylene glycol as solvent, mixed and refluxed in the presence of nitrogen protection and heating, added water solution after refluxing, adjusted pH value with hydrochloric acid, extracted with n-hexane, washed with brine, dried with magnesium sulfate, and finally concentrated under reduced pressure, the mixture of conjugated linoleic acid can be obtained The process of this method is simple, the product is nontoxic and easy to deal with, so it is widely used It is suitable for the production of conjugated linoleic acid from vegetable oil rich in linoleic acid, such as soybean oil, safflower oil, cottonseed oil, corn oil, etc its products can be widely used in food processing (2) Oleic acid allyl alcohol dehydration oleic acid allyl alcohol is a kind of oleic acid allyl alcohol with 8-hydroxy or 11-hydroxy after it is reduced to peroxide by light Allyl oleate is a secondary product of oleic acid, which exists in natural food It can be dehydrated to conjugated linoleic acid under the condition of acid catalysis (BF3, HCl) The conjugated linoleic acid was methylated by the specificity of G candidum lipase Because the lipase has the specificity for cis-9, trans-11-octadecadienoic acid, it can be methylated, but trans-10, cis-12-octadecadienoic acid has no effect High purity cis-9, trans-11-octadecadienoic acid can be obtained by molecular distillation technology (3) The castor oil was dissolved in dichloromethane and methanol solution by esterification, and then esterified under the condition of sodium and reflux to form methyl ricinolate The purified methyl ricinolate can react with 12 methylsulfonyl chloride to form 12 methylsulfonoxyoleate methyl ester The toluene solution of 12 methylsulfonoxyoleate methyl ester can be refluxed under the catalysis of DBU (1,8-diazabicyclo [5.4.0] undecanone-7-ene) The main products of complete digestion are cis-9, trans-11-octadecadiene methyl ester Conjugated linoleic acid with high purity can be obtained by urea separation and purification (4) The microbial fermentation method is to transform linoleic acid into conjugated linoleic acid by using linoleic isomerase in microorganisms From the current research results, lactobacillus is a relatively ideal strain, especially acidophilic lactobacillus can effectively change linoleic acid or glycerin linoleic acid into conjugated linoleic acid Lactobacillus contains linoleic isomerase, and its action site is C12 double bond of fatty acid instead of C9 double bond, so it can convert linoleic acid into cis-9, trans-11-octadecadienoic acid instead of other isomers, and the conjugated linoleic acid can be directly extracted from cell culture solution The above is the introduction of the synthesis method of conjugated linoleic acid If you need to know more about conjugated linoleic acid, please continue to pay attention to the food safety common sense column of Baibai safety net Editor in charge: Wang Xiaoli