Synthesis of solid-phase peptides.

this experiment by aniline and acetic acid by high temperature dehydration and the production of alamide, due to the high reaction temperature, will cause the reaction raw material aniline oxidation, so the reaction added a reducing agent metal zinc to inhibit the high temperature oxidation of aniline.
In this experiment, the product is purified by water crystallization, crystallization is the simplest, effective and economical means of purification, crystallization often has a small amount of flocs insoluble, resulting in the addition of too many solvents to form an unsaturated solution, so that the crystal yield is low or non-crystallization. For this purpose, the crystalline solution needs
filtering
to remove floc-like insoluble impurities, and for cold crystallization solutions need to be insulate and filtered. In order to get good crystallization, it is often required to keep the crystal insulation crystal, crystallization time is longer.
is an important co-priced bond that forms
protein
in living organisms, and is an important synthesis method for
amino acids
synthesized
peptides
and protein
compounds
. The methods used in this experiment are harsh and do not apply to such purposes.
current methods of synthesis of peptides mainly include liquid-phase synthesis and solid-phase synthesis. In liquid-phase peptide synthesis, the product needs to be separated and purified or crystallized after each peptide to remove unresponsive raw materials and by-products. This step is time-traveling and cumbersome, and the cost of operation is often significant.
in order to reduce this hassle and expect to automate the peptide reaction, Merrifield developed a successful solid-phase peptide synthesis method in 1963, in which he obtained the crystalline LeuAlaGlyVal four peptides. At the same time, Letsinger and Kornet also used solid-phase vectors to synthesize LeuGly in different chemical ways.
has since been continuously improved and refined by Murrayfield himself and other laboratories, making it a common technique in peptide and protein synthesis to this day. For this reason, Murrayfield won the 1984 Nobel Prize in Chemistry.the main design idea of
solid phase synthesis is: first, to synthesize the peptide chain of the end of the amino acid of the niobium base with the structure of the co-priced bond with an insoluble polymer resin, and then combined with the solid phase carrier amino acids as amino components after stripping the amino protection and with excessive active carboxyl component reaction to the long peptide chain.
this step can be repeated many times, i.e. shrinking - washing - to protect - to medium and wash - the next round of shrinking, and finally to the length of the peptide chain that needs to be synthesized.
solid phase synthesis reaction is based on liquid phase synthesis.
Because the most commonly used in liquid phase synthesis is the treatment of peptides in the form of pyrethroide- and solid-phase synthesis, it is also used in solid-phase synthesis to hang amino parts on solid-phase resin and elongate the peptide chain to the ammonia end, as this is conducive to the use of excessive active pyrethroide composition to promote the shrinking reaction close to complete, and excessive niobylate composition is also due to its presence in the solution, can be easily removed by filtration and washing methods.
polymer carriers used in peptide synthesis are mainly four types. That is, different cross-linked polystyrene-styrene cross-linked resin;
.