takara product question and answer - modified enzyme question and answer.

1, Ligases

Q-1: comparison of various connective enzyme properties.

Q-8: How is the carrier dephosphate reaction detected?

III, DNA Polymerase

DNA Polymerase

Q-1: a comparison of the characteristics of various DNA.

Q-2: Klenow Fragment's leveling reaction system and reaction conditions.

Q-3: What's the difference between Klenow and T4 DNA Polymerase?"

Q-4: Klenow is not very efficient after the repair of the end of dna, why?

Q-5: What is the relative activity when you want to use Klenow at 16 to 22 degrees C?

Q-6: When Klenow is used for DNA repair, DNA seems to be broken down in small amounts, why?

Q-7: Why is it not efficient to end tag with Klenow and ddNTP?

Q-8: Do you want to cut 3' protruding ends into flat ends with T4 DNA Polymerase?

Q-9: Can phosphate-based DNA fragments at the end of a 3' depression produced by ultrasound treatment be repaired with T4 DNA Polymerase

reretrovirase

Q-10: The difference between Each Reverse Transcriptase.

Q-11: What is the reason why long-chain cDNA cannot be synthesized?

TDT

Q-12: What should I pay attention to with TdT enzymes?

4, RNA Polymerase

Q-1: What should I be aware of when using PolymerRNAase?

Q-2: Do I need to do DNase I (RNase free) processing after the reaction ends?

5, Nuclease

Q-1: Comparison of features of various Nuclease.

Q-2: Precautions for the use of various nucleases.

Q-4: Mung Bean Nuclease after THE DNA electrophoresis strip blurred, is it cut into (Nibbing)?"

Q-5: Added a high concentration of 600 mM to the reaction fluid of BAL 31 Nuclease, does it have any effect on the reaction?

Q-6: BAL 31 Nuclease also has an effect on Nick?

Q-7: What if you just want to remove a few nucleotides from the end and cut them with the usual reaction

Q-8:Exo III is not specific to Nuclease contamination when electrophoresis occurs when it is electrophoresis after DNA

Q-9: Notes on the use of DNase I (RNase Free).

Six, DNA Topoisomerase I, T4 Polynucleotide Kinase, Ribonucleaseaseor

Q-1: What are the issues to be aware of using DNA Topomer.

Q-2: T4 Polynucleotide Kinase Catalytic 5' End Marker Reaction Is Efficient?

Q-3: T4 Polynucleotide Kinase indignity method.

Q-4: Can you use γ-35S instead of (γ-35P) ATP when marking DNA with T4 PNK?

Q-5: What are you aware of when using Ribonuclease Addor?"I, Ligases

Q-1: comparison of various enzyme properties.

A-1:

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