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Telomere Length Analysis by Quantitative Fluorescent In situ Hybridization (Q-FISH)

 Last Update: 2020-12-08
 Source: Internet
 Author: User

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Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high-affinity probes for telomeric repeated sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.

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