Tens of thousands of female eyelid cells "confide" the secrets of aging

fairy water, puffed water, aurora water... The new concept of age-reducing artifacts, round after round; anti-blu-ray, triple anti-wrinkle, intensive repair... Which of these features is the most powerful?
whether it's a light-haired woman or a "white bone essence", the biggest concern for women is always the same - what determines the state of youth?
November 25th, Chinese scientists published a research paper in the journal Developmental Cells, saying they had answered this key question with the most accurate biotechnology possible.
"We sequenced 35,678 eyelid skin cells by single cells and obtained about 530 GB of data. Zhang Weixuan, one of the authors of the paper
and a researcher at the Beijing Genomics Research Institute in China, told Science and Technology Daily that they worked with Liu Guanghui of the
Animal Research Institute of China and Qu Jing Research Group to find the difference between young skin cells and senescing skin cells at the molecular level from tens of thousands of cells by analyzing gene expression data obtained by single-cell sequencing technology." in the skin of the whole body, the aging of the skin of the face is very obvious. Zhang Weiwei explains that in the process of aging, the eyelids are the most obvious. The aging of the eyelid skin is the "vanguard" and "microcosm" of the aging of the whole body.
" studies have shown that AI face recognition models include eye skin recognition models that can predict a person's age more accurately by using face recognition methods. Zhang said.
, the team chose human eyelid cells as their subjects in a number of human cells. "Our sample came from the plastic surgery hospital's heavy blepharitis." This allows research on aging to be conducted directly on human cells, Zhang said, while most previous studies have been based on experimental animals, making it difficult to accurately reveal the laws of aging in the human body.
took the team a long time to obtain skin samples of the eyelids of young, middle-aged and older people. "Through traditional dyeing methods, we have demonstrated that there is a clear age difference between these skin cells." Zhang said that cells of three age groups have a series of aging changes in skin thickness, collagen density and so on.recent years, the accuracy of single-cell sequencing technology has increased, allowing people to understand what is happening in a small cell.
the cells together, they converse into a map that covers all the gene expression of the cells. The map consists of big information data for each cell, and the "alienation" of any cell can be perceived.
single-cell technology can "find out" changes in cellular and molecular levels during human skin aging, and the researchers have developed gene expression maps of multiple skin cell types of different ages.
skin cells, including skin substrate cells, ratchet cells, granulocyte cells, melanin cells, dermatical cells, etc., have their own 'portraits'. Just as face recognition uses big data about facial information, cell "portraits" use its molecular expression, Zhang said.
, "We found that skin substrate cells rich in skin stem cells varied the most between individuals. Zhang said the state of stem cells is closely related to the young state, and the skin substrate cells are divided into six subpoptons because of their high heterogeneity.
data analysis showed that the skin of the middle-aged group (over 40 years old) and the older group (around 70 years old) had more similar transcription group characteristics, and the skin cell characteristics of the younger group (around 20 years old) were significantly different.
" suggests that the eye skin has aged in middle age. Although not visible to the naked eye, gene expression is characterized by aging. Zhang said that the gene expression of a variety of aging skin cells showed a decrease in DNA repair capacity, increased damage to biological molecules and so on.this is the first international report on the aging of the skin of the human eye single-cell transcription map. In order for the academic community to recognize the aging symptoms of "focusing", scientific research also needs complete closed-loop verification.
"We've seen some expressions down in the single-cell map of experimental cells, so did this happen to other people?" For this reason, the team also demonstrated that the same changes occurred in the eyelid samples of 13 other people as aging intensified, Zhang said.
the study, which was excavated and reverse-validated with bioinfossic data, found that the reduction of two growth factors, KLF6 in substrate cells and HES1 in dermatical fibroblasts, could trigger skin aging.
"We blocked the expression of KLF6 and HES1 in the cells we cultured, respectively, and the results showed that the aging of these cells was accelerated, which in turn activated expression to slow cell aging." Zhang said.
with a "scale" to judge cells young and old, the team cultured eyelid skin cells in-body and selected substances from a pool of bioactive small molecules to see if they could slow aging.
"We tried different small molecules such as vitamin C, metformin, quercetin, and finally the natural compound quercetin was the most stable and efficient, and different experiments at the cellular level proved that it can delay the aging of human leather fibroblasts. Zhang said.