Terranean staining of bacteria

Terrain dyeing is one of the most important and widely used methods for bacterial identification. According to the dyeing results of this method, bacteria can be divided into two major categories. One type of bacteria is able to retain the initial dye (crystallization, etc.) in the cell, not affected by the decolorizer (alcohol), and finally the bacteria are dyed purple or dark blue called Gram-positive; There are also
strains
the 1950s, and Grady's staining is variable.
the principle of
dyeing, and master the operation technique of Terran's dyeing method.
The
is an important identification staining method widely used in bacteriology. Founded in 1884 by the Danish physician Gram.
bacteria are first crystallized by alkaline dyes, then by iodine-based dye (to increase the affinity between dyes and cells), then decolorized with alcohol or acetone, and then dyed with a complex dye. Those who are not decolored and keep the original color are Terratin-positive bacteria (G-plus); This method can divide bacteria into two categories.
Causedge's staining is mainly the use of two types of bacteria cell wall composition and structure is different. The cell walls of Glollaton-negative bacteria contain more lipids, while peptide polysaccharides contain less. When decoloring with ethanol or acetone, lipids are dissolved, increasing the permeability of the cell wall, making the crystalline purple and iodine complex after initial dyeing easy to seep out, resulting in the cells being decolorized, after re-dyeing, and then dyed with the color of the re-dyeing solution; Grady-positive bacteria cell wall peptide polysaccharide content and cross-linking, lipid content is small, after ethanol or acetone washed away, peptide polysaccharide layer of the aperture becomes smaller, reduced permeability, therefore, the cell still retains the color at the time of initial infection.
( Experimental Materials, Medicines and Appliances)
1, E. coli
Bacillus Subtilis
Staphylococcus aureus
2, dyed ammonium glyphosate crystalline amethyst
Grain's iodine
95% alcohol
sedated red liquid

3, dish washed slide 6 pieces,
microscope
alcohol lamp, inoculation ring, balsamic asphalt, xylene, mirror paper, absorbent paper, tweezers, glass cylinder, bottle Distilled water 1, take
culture
12-24h E. coli, Spoore spores and Staphylococcus aureus smeared and fixed respectively.
2, add ammonium glyphosate crystalline a drop of amethyst, dyeing for 1 minute.
3, pour out the dye and gently rinse
with
bottle and water.
4, iodized liquid dyed for 1 minute, washed, and then dried with absorbent paper.
5, tilt the slide slightly, drop 95% alcohol decoloration (wash until there is no purple in the flowing alcohol), and wash.
6, add sashum red re-dyeing, dyeing for 30 minutes, washing, using absorbent paper to absorb dry or dry.
7, with oil mirror mirror observation.