The advanced structure of chromatin is systematically studied by influencing the expression of the WUS gene.

All tissues and organs of higher plants are derived from the dyscosle tissue, and the WUCHEL gene is the key gene for the maintenance and termination of plant biological tissue.
WUS expression regulation is a complex network, but its specific regulatory mechanism is still unclear.
more and more studies show that the advanced structure of chromatin plays an important role in regulating gene expression.
Liu Xigang Research Group of the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, using molecular biology, molecular genetics, biochemistry and cell biology as research materials, systematically studied the advanced structure of chromatin by influencing the expression of WUS genes and regulating the molecular mechanism of the maintenance and differentiation of the flower ingenuity tissue.
studies have shown that the two side areas of the WUS gene combined with AG and TFL2 have been found to form a chromatin ring in space through chromatin capture technology (3C) and combined with chromatin immunoprecipitation (ChIP-3C).
AG promotes the formation of this chromatin ring in the development of flowers, and the formation of the WUS chromatin ring is not affected by the distance and sequence between the two regions.
further studies show that AG and TFL2 interact in plants, and TFL2 is dependent on AG in combination with chromatin rings.
using THE GENETICALLY modified plants that knocked out the WUS CArG box region using CRISPR/Cas9, the study found that chromatin rings inhibited the expression of WUS by blocking the recruitment of RNA polymerase II on THE WUS.
this study, this study finds a new mechanism for the regulation of WUS expression, and provides a new clue for subsequent studies to promote the formation and change of chromatin spatial structure.
's findings were published online April 16 in The Plant Journal.
title a Chromatin Loop Represses WUSCHEL Expression in Arabidopsis. Guo Lin, an associate researcher in the Liu Xigang Research Group at
, Cao Xiuwei, a master's student, and Liu Yuhao, a doctoral student at Fudan University in Shanghai, were co-authors of the thesis.
the research was funded by the National Natural Science Foundation of China, the National Key Basic Research and Development Program (the "973" Program) and the "100-person Program" of the Chinese Academy of Sciences, as well as key laboratories for plant cells and chromosomal engineering.
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