The agricultural fungal toxin prevention and control innovation team of the Chinese Academy of Agricultural Sciences analyzed the molecular mechanism of the difference between aflatoxin and toxicity in different food substrings

Original title: Chinese Academy of Agricultural Sciences agricultural fungal toxin prevention and control innovation team to analyze the molecular mechanism of aflatoxin in different food substations of toxic differences
aflatoxin is a common rotting fungus, infected with peanuts, corn, rice and other common crops, and in the storage and processing process to pollute a variety of
fedal food
and feed. The secondary metabolite
aflatoxin
produced by aflatoxin is a class of carcinogens, and up to 28% of primary hepatocellular carcinoma cases worldwide are caused by aflatoxin
toxins
and aflatoxins pose a serious threat to human health. In addition, the problem of aflatoxin exceeding the standard has also become China's food and
agricultural products
the EU's biggest obstacle. Therefore, the study of aflatoxin synthesis control mechanism, prevention and control of toxin pollution is to ensure food safety, stable export trade of the country's major needs.
early reports indicate that aflatoxin's ability to produce poison on different food substrings is different, but its mechanism is not analyzed. In this study, aflatoxin was cultured on three different food substations: peanuts, corn and rice, and aflatoxin yield was detected, and the transcriptional expression level and protein expression level of aflatoxin were analyzed on different substations using comparative transcription group and
protein
group technology. The results showed that aflatoxin yield was significantly higher in corn and rice liquid media than in peanut liquid media, histological analysis and qPCR testing revealed the initial steps of aflatoxin synthesis
gene
aflA, aflB, aflC, and substrate synthesis gene accA significantly increased in corn and rice media; Compared with peanuts, in corn medium culture, acetyl coenzyme A synthesis and accumulation of related genes in aflatoxin a large number of upward, resulting in acetyl coenzyme A consumption and utilization of the relevant genes reduced, so the corn substrate led to a large accumulation of toxin synthesis substrate
acetyl coenzyme
A; Based on the above conclusions, it is speculated that different food substrates affect aflatoxin synthesis from the substrate supply level, and the difference of carbon source type (starch content) in different food substrates is the most important reason for the difference of aflatoxin production. This study analyzes the differences between aflatoxins in different food substations from the genetic and protein levels, and lays a theoretical foundation for the
dispication warning
and prevention and control of aflatoxins and toxins.
this article was published in July 2020 in the journal Fro
ntier in Microbiology (Region 2, 4.235), and the study was funded by the 13th Five-Year Plan National Key Research and Development Program and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
article Source: