THE APPLICATION OF LB TECHNOLOGY IN THE FIELD OF PHOSPHOLIPID HYDROLYSIS CATALYSIS.

LB technology is a method of studying matter at the molecular level, and LB technology is widely used for its easy preparation of ultra-thin, orderly single-molecule membranes and the ability to accurately control thickness.
LB technology can dissolve the surface-active sex molecules (phospholipids) in water can automatically form directional adsorption and form a single molecule film in the interface, typical phospholipid molecules have a similar head-to-tail structure of the polar base and non-polar base, when the surface pressure increases, will produce a single molecular membrane state change, that is, from gas to liquid expansion and intermediate state, further compression, into a liquid condensation state, phospholipids tightly arranged.
from the perspective of interface chemistry on the interface chemical properties of lipase research, can be a good understanding of the interface-non-homogeneous interface catalytic hydrolysis activity performance, the use of LB membrane analysis technology to carry out lipase for the formation of phospholipid monomolecular layer at the gas interface hydrolysis performance research began to be many researchers concerned, and actively carry out research in this area.
enzyme is an efficient biocatalyst with special catalytic functions, which not only catalyzes chemical reactions in organisms, but also informs chemical reactions in non-invivals, and can catalyze reactions that are difficult to complete by chemical methods. Compared with chemical catalysts,
has the advantages of strong substrate specificity, high catalytic efficiency and mild reaction conditions.
lipase (trikyriglycerides) is common in plants and animals and microorganisms, and has a wide range of applications as biocatalysts in organic synthesis.
it is an important ester-bond hydrolysis enzyme, can hydrolyse glycerin and fatty acid formation of ester bonds, so also known as triamide glycolycolyse.
lipase can catalyze hydrolysis and synthesis of glycoelcompounds, in organic phases, can also catalyze esteration, transesters, alcohols, acidsolutions and amino solutions, the catalytic substrate can be esters, acids, alcohols, acid sacline and amide.
the catalytic mechanism of lipase, that is, the mechanism of "interface activation", compared with other hydrolyzed enzymes, lipase can show high catalytic activity on the hydro-oil interface.
enzyme deglue technology is the use of lipase to solve phospholipid hydrophospholipids, compared with natural phospholipids, hemolytic phospholipids retain the molecular structure of the two parents, reduce non-polar groups and oxidized unsaturated double-bond structure, improve its hydrophilic and stability, when the water can form a liquid hydrated crystal, after the process of oil-water separation to achieve the reduction of phosphorus content in oil.
as a new degluing method, enzyme degluing because of its high degluing efficiency, simple operation without pollution, neutral oil loss and other characteristics have been widely concerned, enzyme degluing not only can improve the degluing rate of grease, but also its grease by-product - enzyme modified phospholipid has a great use of value, to achieve efficient use of resources. Wang Yonghua team of the School of Food Science and Engineering of South China University of Technology in
(European Journal of Science and Technology, 2017, 119 (3), 1600175-1600180) used a single molecular lamine technology to study the hydrolysis activity of Lecitase Ultraenzyme for the formation of a single molecular layer of phosphades in the gas fluid interface, and found that Lecit Ultrasse enzymeshow showed better hydrolysis performance on PE, PC, AND PS.
used a combination of LB membrane analysis techniques in the study of catalytic activity of enzymes, and analyzed the hydrolysis properties of lecitase Ultra10 phosphatase by analyzing the membrane pressure changes in the monolith single-molecule film, so as to better understand the application principle of this phosphase in the technology of degluing grease, and provide better theoretical support for the future use of vegetable oil enzyme degluing technology.
LB membrane technology has become an effective method to study and improve enzyme activity, because it has the advantages of precise control of membrane thickness, molecular nanostructure and molecular orientation. Based on the activation characteristics of lipase interface, the
applied LB membrane technology to study the catalytic hydrolysis properties of lipase, so as to understand the catalytic hydrolysis mechanism of lipase and open up the application prospect of the technology in bioenzyme catalysis.
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