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    Home > Biochemistry News > Microbiology News > The application scope of microbial fermentation engineering, the operating procedures of microbial fermentation system.

    The application scope of microbial fermentation engineering, the operating procedures of microbial fermentation system.

    • Last Update: 2020-07-28
    • Source: Internet
    • Author: User
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    The level of microbial fermentation production mainly depends on the genetic characteristics and culture conditions of the species themselvesThe application scope of fermentation engineering is: pharmaceutical industry food industry energy industry chemical industry agriculture: modified plant genes; environmental protection and so onThe following small compilation for you to introduce the application scope of microbial fermentation engineering and microbial fermentation system operating proceduresThe application range of microbial fermentation engineering, including cider, beer, liquor and other wines, is the use of brewing yeast fermentation under anaerobic conditions to convert glucose into alcohol productionLiquor is distilled so the main ingredients of liquor are water and alcohol, as well as some heated volatile substances such as various esters, other alcohols and small amounts of low-carbon aldosterone compoundsFruit and beer are non-distilled wine fermentation when yeast in fruit juice or fermentation liquid glucose into alcohol and other nutrients will be partially used by yeast to produce some metabolites such as amino acids, vitamins and so on will also enter the fermented liquorTherefore, the nutritional value of fruit and beer is higherThe other aspects of the vinegar sold in the food store or supermarket are produced by the fermented fermentation of the bacteria acetic acid in the chemically synthesized food grade acetic acid, which converts the alcohol produced by solid fermentation into acetic acidDue to the difference sacs or curvatures used in glucose fermentation, lactic acid or other organic acids are produced, thus making vinegar have different flavorsSoy sauce soy production to soybeans as the main raw material of other wheat bran, wheat, corn, etcthe above raw materials are crushed into solid culture medium in aerobic conditions using protease-producing mold such as black crest mold fermentationIn the course of growth, microorganisms produce a large number of protease that hydrolyze the protein in the medium into small molecules of peptides and amino acids and then wash and adjust to make soy sauce productsSoy sauce is rich in amino acids and peptides with special flavorsYw ys milk under anaerobic conditions by lactic acid bacteria fermentation to break down lactose and further fermentation to produce lactic acid and other organic acids, as well as some aromatic substances and vitamins, etc.; Therefore, yogurt is rich in nutrition, easy to digest less lactose is suitable for people with lactose inadaptation to the excellent food""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""""" Root mold grows with a large amount of amylase that delyssizes the starch in the rice into glucose and ferments some glucose to produce alcoholBecause of the use of different root mold can produce different alcohol, different sweetness and different scentofsBread bread is the use of active dry yeast (bread yeast) activated and flour mixed fermentation and then added a variety of additives by roasting production After the flour fermentation, the starch structure changes and becomes easy to digest and the nutrients easy to absorb Candy, biscuits, jelly, etc added red color to adjust the color, fruit juice, biscuits, bread, snacks, instant noodles, etc added jaundice suspension, stability, thickening, improve taste, prevent sticky teeth, extend storage period, etc , all kinds of canned items include vegetables, fruits, mushrooms, fish, meat, meat, Egg canned sausage packaging milk, etc added lactobacillus peptides to preserve, anti-corrosion to preserve nutrients and improve taste, etc ; Microbial fermentation system operating procedures """"Pre-fermentation preparation check whether the power supply is normal air compressor, microcomputer system and circulating water system is working properly Check that the valves, connectors and fastening screws on the system are tightened The pressure of the air compressor to check the seed tank, fermentation tank, filter, line, valve and other seals are good for leakage Whether the tank jacket is sealed (with emphasis on inspection during the season) ensures that all valves are closed (except for valves in front of the solenoid valve) Check whether water (cooling water) pressure, voltage, gas (vapor) pressure can be supplied normally The inlet pressure maintenance should not exceed the temperature at the permissible range should be lower than the fermentation temperature , the single-phase power supply , the frequency tank is reliably grounded , the input steam pressure should be maintained after entering the system, the air input pressure value air entry pressure should be controlled (air primary filter pressure value) Temperature, dissolved oxygen electrode, electrode correction and calibration are detailed on the touch screen, calibration help Check that each motor is operating properly (in total) Whether the solenoid valve can be sucked in properly (the complete system is a total solenoid valve) After the ""sterilized fermentation system is installed in the first cleaning tank cleaning: acid-base tank, feed tank, seed tank can be the cylinder above the flange removed by the operator using a clean cloth after manual cleaning discharge of the sewage in the tank in more than a few times can be flushed The cleaning of the fermentation tank can be flushed by a water pipe through a hand hole to the wall of the tank when the water level rises to the second impeller of the mixing shaft to stop the flushing motor stirring cleaning The cleaning of each line can be first rinsed with clean water and then according to the corresponding function of the corresponding cleaning medium (cleaning the pipeline should be to protect the various components in the pipeline as a prerequisite) the specific steps can refer to "the sterilization of the air line." If the fermentation system for a long time not used or cultured bacteria and the previous batch is not the same time can be used to clean other tanks can also use the fermentation tank cleaning method can be used to deal with the fermentation system sterilization after cleaning The sterilization filter on the air line air line uses steam through the pressure relief valve (the air relief valve cannot be steam-sterilized so the air pre-filter is not sterilized), the steam filter and then into the sterilization filter The filter element of the air exorcisaing filter cannot withstand high temperature and pressure so that the steam relief valve must not exceed The exhaust valve at the lower end of the sterilizing filter during the emptying process should be slightly opened to remove condensate The empty time should last about a minute when the equipment is first used or after long-term use after start-up, it is best to use intermittent emptying that is the first empty after the interval - hours empty again in order to eliminate spores After the air-cancelled filter should be air-blow-drying for about a minute and then the air path valve should be closed Keep the air ducts positive The fermentation tank empty (seed tank, filling tank, acid-base tank empty as a reference basis) the fermentation tank should open the sewage valve before emptying (so that the pressure inside the jacket does not exceed pressure) Close the micro-open steam valve open to the tank to open the steam; The valve on the inoculation outlet exhaust valve and sewage line on the tank, micro-opening so that steam through these valves discharge when the humidity reaches the start of the time adjustment valve, the opening to maintain the temperature of the tank - (pressure is generally in-) according to the process can adjust the temperature and pressure of the empty When the time is reached - close, then close again after minutes, open the valve on the air line, cool the air in the tank to keep the tank positively pressed between When rapid cooling is required, close the jacket sewage valve open the cooling water valve to the jacket water cooling to the jacket after reaching normal temperature Intermittent emptying can be used in special cases (interval-hour-empty-once-in-an-hour to eliminate spores) Dissolved oxygen and electrodes are removed and properly preserved to extend their service life Fermentation tank elimination (seed tank, filling tank, acid-alkali tank as a reference basis) is the process of sterilizing the medium with steam when the medium is added to the tank After the empty end of the closure of the intake valve open discharge tank pressure will be corrected, electrodes installed as soon as possible the matching medium from the feeding port added to the tank The formula quantity of the medium should be pre-empted before entering the tank should be determined according to the process requirements as the final volume of the fermentation solution for the total volume of the tank% or so calculation (the medium of foam more than % or so foam less can reach -%) taking into account the condensation and inoculation factors and whether the flow plus the initial set capacity is determined by the production according to the requirements of the process to explore in practice Turn on the mechanical agitator at low speed so that the material in the tank is evenly mixed Open the jacket sewage valve, steam valve to warm up the inner medium (preheating with the jacket steam) When the temperature in the tank rises to the temperature of the steam (the specific temperature should be changed according to the steam quality) close the jacket inlet valve close full-open steam open full open through the sampling port to the tank through the steam shut-off, full open micro-open to the tank through the steam micro-open exhaust valve (three-way steam sterilization) to close the motor stirring When the temperature rises to - C tank pressure to control the steam valve 、、、 the open temperature and tank pressure and start timing micro-opening flame inoculation port outward steam when the time reaches the minute before the closure of the flame inoculation port closed (before closing should artificially open large steam several times to avoid the remaining of the medium at the valve) closed, closed, stop the supply of steam Close the jacket sewage valve Open the cooling water valve to the jacket water cooling open motor stirring When the pressure drops to open the air valve, to the tank air to speed up the cooling speed and keep the tank pressure until the in-tank culture temperature drops to the inoculation temperature Close the valve to stop the cooling water when the temperature in the tank drops to higher than the temperature required by the fermentation process Seed tank inoculation, transplant vaccination: This system should be prepared in advance before vaccination with flame sealing: alcohol cotton, pliers, tweezers, vaccination rings, asbestos mesh gloves, word wrenches The amount of inoculation in the triangular flask is determined according to the process requirements The alcohol cotton surrounded by the vaccination ring lit around the bacteria bottle mouth in the flame for a while with a word wrench unscrewed the inoculation port quickly pour the bacteria into the tank at this time should be ventilation to the tank so that the inoculation mouth has air discharge (pressure close to zero but not zero) Tighten the inoculation cap after sterilizing on the flame The tank pressure is kept after vaccination - adjusting the amount of ventilation Species transfer: Before species ingesting the condensate in the steam line and keeping the steam valve slightly open (only running water does not drain steam) so that the condensate in the steam line can be drained at any time When transferring operations, special attention should be paid to adjusting the pressure of the differential seed tank between the seed tank and the culture tank should be higher than the culture tank in order to be transferred! When specgearing is closed, the steam valve should be removed immediately to avoid negative pressure pollution Immediately after the completion of the migration, the transfer valve should be closed and then open the steam valve connected with the steam into the steam for steam disinfection and immediately clean the seed tank to prevent the fermentation material from bonding to the pipe valve and tank wall disinfection immediately after the closure of the steam valve and the discharge of condensate valve and tube cap Samples Samples Can be sampled at the sampling port after vaccination is completed or when sampling is to be taken during fermentation Pre-sampling pipeline valves need to be steamed to prevent contamination of the bacteria and misleading sampling is also needed to flush the sampling pipe and valve with steam after the end of sampling How to operate: full-open steam valve micro-open when the time to minutes will be alcohol cotton around the sampling mouth lit close open first the sample tube liquid drained first ready to open the sampling bottle on the edge of the flame quick sampling and cover closed and then open the cleaning sterilization sampling port minutes off, After sampling is completed, the value of the fermentation solution should be detected by the instrument and then corrected, and when the system is inoculated to adjust the pressure and flow rate, the system one can enter fermentation mode for automatic control (temperature, and, ) the specific process parameters by the operator himself set the system will be set in accordance with the set parameters can be automaticcontrol or manual control by the operator After the completion of the fermentation culture sampling to use the instrument to detect the value of the fermentation liquid and then correct, when the system after the completion of the injection of pressure and flow adjustment system can enter fermentation mode automatic control (temperature, , ) specific process parameters according to the production requirements of their own set system according to the set parameters can be automatic control or operator manual control The general speed of the temperature-controlled culture tank pressure after the seed is transferred into the seed tank or fermentation tank is regulated according to the process requirements to adjust the temperature of the circulating water to control the fermentation temperature when the ambient temperature is higher than the fermentation temperature, and when the temperature of the medium is lower than the fermentation temperature, heating with hot water The adjustment is realized by the control system by automatic addition and subtraction by the executing mechanism The foam alarm is detected by the foam probe as a foam signal on the touch screen as an indicator Note: Before sterilization, the acid bottle base bottle seisquid in the steriliser for control during electrode correction culture using the peristaltic pump to achieve acid and alkali Measurement and control of dissolved oxygen () dissolved oxygen electrode: the dissolved oxygen value of the dissolved oxygen at a constant fermentation temperature before vaccination is used as % Dissolved oxygen measurement and control during culture: the control of the value uses the control of the air flow and the speed of the adjustment to achieve the control of the association between the speed and the dissolved oxygen Second, the intake (manual) control must be adjusted simultaneously The outcoming material of the """ materialist is the use of tank pressure to discharge the fermentation liquid from the discharge pipe according to the concentration of the fermentation liquid tank pressure can be controlled in ——。 After the discharge of dissolved oxygen, instrument for cleaning and maintenance Immediately after the discharge of the fermentation tank and the pipe valve seisker, and open the air compressor to supply gas to the fermentation tank and stir to flush the fermentation liquid in the pipeline clean If the fermentation tank is not used for the time being, the fermentation tank is emptied and the water in the tank, the jacket and the pipe is emptied (The first quarter of Zhengzhou Zhongguang Environmental Technology Co., Ltd launched a new product: bio-insecticidal microbial species - white bacteria)
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