The carrier binding method of enzymes is fixed by the method of interlinking and burying.

methods of fixing enzymes are not less than 100 kinds, which can be summarized into three categories, namely carrier binding method, cross-linking method and burial method.(i) carrier binding method refers
method of fixing enzymes to non-water-soluble carriers. According to the different fixed methods, this method can be divided into physical adsorption method, ion binding method and co-price combination method. Physical adsorption method refers to the method of adsorption of enzymes to the surface of solid adsorbents, which are mostly activated carbon and porous glass. Ion binding method refers to the method of binding enzymes to non-water-soluble carriers with ion exchange groups by ion bonds, such as ion exchange resins. Co-price binding method refers to the method by which enzymes and vectors are combined in the form of co-price bonds, which requires enzymes and vectors to have a combination of amino, carboxy or hydroxyl.(ii) cross-linking method
translinking method refers to the method of connecting enzymes and enzymes into a mesh structure by means of dual-functional
-reagents
. The crosslinking agent used in the crosslinking method is a water-soluble compound such as
,
.(iii) encasing method
encasing method refers to enzymatic enzymatic encasing in a porous carrier. For example, enzymes are wrapped in polymer gels such as polyacrylamide gels or in semi-permeable polymer membranes such as nitrate cellulose. The former is buried in a grid type, while the latter is buried in a microcapsules type.
with the fixed enzyme technology is the enzyme bioreacter. A container fitted with a fixed enzyme material is an enzyme bioreacter, which is an important production workshop for turning reactive substances into products. If the glucose solution slowly flows into a bioreactive device filled with glucose isomerase, it comes out of a new liquid that is much sweeter than the original solution.
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