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The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites

 Last Update: 2021-02-14
 Source: Internet
 Author: User

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comprehensive metabolic profile

natural food products

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Filamentous fungi have long been recognized to be a rich source of secondary metabolites with potential medicinal applications. The recent genomic sequencing of several
Aspergillus
species has revealed that many secondary metabolite gene clusters are apparently silent under standard laboratory conditions. Several successful approaches have been utilized to upregulate these genes and unearth the corresponding natural products. A straightforward, reliable method to purify and characterize new metabolites therefore should be useful. Details are provided herein on the cultivation of
Aspergillus nidulans
and the LC/MS analysis of the metabolic profile. Following is an explanation of silica gel chromatography, HPLC, and preparative TLC. Finally, the
NMR
characterization of previously unknown
A. nidulans
metabolites is detailed.

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