-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
- Cosmetic Ingredient
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Recently, "Science Bulletin" published the article "The molecular mechanism of new coronavirus binding to pangolin ACE2 receptor" by the research team of the Institute of Microbiology, Chinese Academy of Sciences.
This article analyzes the new coronavirus (SARS-CoV-2) and two pangolin coronaviruses (pangolin-CoVs, GX/P2V/2017 and GD/1/2019) combined with human ACE2 (human ACE2, hACE2) and pangolin ACE2 ( The ability of pangolin ACE2, pACE2) analyzed the crystal structure of SARS-CoV-2 RBD and pACE2 complex, and further simulated the binding of GX/P2V/2017 RBD and GD/1/2019 RBD to pACE2 and hACE2 receptors, respectively.
It helps us better understand the evolution of SARS-CoV-2, warns us that pangolin coronavirus has the potential to infect humans, and emphasizes the importance of continuous monitoring of pangolins carrying viruses to prevent the virus from spilling out and causing human diseases.
Coronavirus disease 2019 (COVID-19) is the third human epidemic caused by coronavirus after SARS and MERS.
The current epidemic situation is still severe.
Its pathogen is COVID-19 virus, also known as SARS-CoV -2 or HCoV-19.
Research suggests that SARS-CoV-2 may have originated from bats, but the mystery of its intermediate host has not yet been solved.
On the basis of long-term research on the invasion of coronavirus, the research team quickly discovered that SARS-CoV-2 used ACE2 as an invasion receptor at the beginning of the epidemic, and analyzed the molecular mechanism of SARS-CoV-2 RBD binding to hACE2 (Wang et al.
, Cell, 2020).During the period, animal infection experimental data and epidemiological investigations showed that SARS-CoV-2 can infect cats, dogs, tigers, minks and other animals.
The research team explored the potential host range of SARS-CoV-2 and found that SARS-CoV-2 can bind to the ACE2 of a variety of animals, including domestic animals, pets, and wild animals, suggesting that these animals may be SARS-CoV-2 The potential host also excludes the possibility of some animal infections, providing important clues for the traceability of the virus (Wu et al.
, Cell Discovery, 2020).
At present, apart from bats, pangolins are proven to be the only mammals that carry SARS-CoV-2 related coronaviruses.
The viruses that carry them belong to two unique branches, namely Guangdong pangolin coronavirus (GD pangolin-CoVs) and Guangxi pangolin coronavirus (GD pangolin-CoVs) and Guangxi pangolin coronavirus.
The genomic sequence homology of the virus (GX pangolin-CoVs) and SARS-CoV-2 is 92.
4% and 85.
5% respectively, second only to RaTG13 (96.
2%) and RmYN02 (93.
3%), but in the S protein RBD region, The amino acid homology between GD pangolin-CoVs and SARS-CoV-2 is as high as 97.
4%, which is higher than that of RaTG13 (89.
2%), which suggests that SARS-CoV-2 may be derived from the recombination of related coronaviruses.
Nevertheless, we still know little about the evolutionary relationship between SARS-CoV-2 and pangolin-CoVs, and the molecular mechanism of SARS-CoV-2 RBD binding to pACE2 and pangolin receptor or human receptor binding of pangolin-CoVs is still Not sure.
In this article, the research team first analyzed the ability of SARS-CoV-2, GX/P2V/2017 and GD/1/2019 to combine pACE2 and hACE2 respectively, and found that SARS-CoV-2 RBD, GX/P2V/2017 RBD and GD/ 1/2019 RBD has similar binding affinity to pACE2 and is about 4 times lower than that of hACE2; the molecular mechanism of the interaction between SARS-CoV-2 RBD and pACE2 is analyzed, and it is found that the binding mode of SARS-CoV-2 RBD to pACE2 is similar to that of hACE2 or hACE2.
Cat ACE2 is similar, but more similar to the binding of hACE2; using homology modeling to further simulate the binding of GX/P2V/2017 RBD and GD/1/2019 RBD to pACE2 and hACE2, respectively, and found that the way they combine pACE2 and hACE2 is similar to that of pACE2 and hACE2.
SARS-CoV-2 RBD is similar and consistent with the affinity data.
SARS-CoV-2 RBD, GX/P2V/2017 RBD and GD/1/2019 RBD are combined with pACE2 and hACE2, respectively.
Comparative article information: [click the link below or read the original text] Wu Lili, Su Jiaqi, Niu Sheng, etc.
New The molecular mechanism of coronavirus binding to pangolin ACE2 receptor.
Chinese Science Bulletin, 2021, 66:73-84, doi:10.
1360/TB-2020-1372
This article analyzes the new coronavirus (SARS-CoV-2) and two pangolin coronaviruses (pangolin-CoVs, GX/P2V/2017 and GD/1/2019) combined with human ACE2 (human ACE2, hACE2) and pangolin ACE2 ( The ability of pangolin ACE2, pACE2) analyzed the crystal structure of SARS-CoV-2 RBD and pACE2 complex, and further simulated the binding of GX/P2V/2017 RBD and GD/1/2019 RBD to pACE2 and hACE2 receptors, respectively.
It helps us better understand the evolution of SARS-CoV-2, warns us that pangolin coronavirus has the potential to infect humans, and emphasizes the importance of continuous monitoring of pangolins carrying viruses to prevent the virus from spilling out and causing human diseases.
Coronavirus disease 2019 (COVID-19) is the third human epidemic caused by coronavirus after SARS and MERS.
The current epidemic situation is still severe.
Its pathogen is COVID-19 virus, also known as SARS-CoV -2 or HCoV-19.
Research suggests that SARS-CoV-2 may have originated from bats, but the mystery of its intermediate host has not yet been solved.
On the basis of long-term research on the invasion of coronavirus, the research team quickly discovered that SARS-CoV-2 used ACE2 as an invasion receptor at the beginning of the epidemic, and analyzed the molecular mechanism of SARS-CoV-2 RBD binding to hACE2 (Wang et al.
, Cell, 2020).During the period, animal infection experimental data and epidemiological investigations showed that SARS-CoV-2 can infect cats, dogs, tigers, minks and other animals.
The research team explored the potential host range of SARS-CoV-2 and found that SARS-CoV-2 can bind to the ACE2 of a variety of animals, including domestic animals, pets, and wild animals, suggesting that these animals may be SARS-CoV-2 The potential host also excludes the possibility of some animal infections, providing important clues for the traceability of the virus (Wu et al.
, Cell Discovery, 2020).
At present, apart from bats, pangolins are proven to be the only mammals that carry SARS-CoV-2 related coronaviruses.
The viruses that carry them belong to two unique branches, namely Guangdong pangolin coronavirus (GD pangolin-CoVs) and Guangxi pangolin coronavirus (GD pangolin-CoVs) and Guangxi pangolin coronavirus.
The genomic sequence homology of the virus (GX pangolin-CoVs) and SARS-CoV-2 is 92.
4% and 85.
5% respectively, second only to RaTG13 (96.
2%) and RmYN02 (93.
3%), but in the S protein RBD region, The amino acid homology between GD pangolin-CoVs and SARS-CoV-2 is as high as 97.
4%, which is higher than that of RaTG13 (89.
2%), which suggests that SARS-CoV-2 may be derived from the recombination of related coronaviruses.
Nevertheless, we still know little about the evolutionary relationship between SARS-CoV-2 and pangolin-CoVs, and the molecular mechanism of SARS-CoV-2 RBD binding to pACE2 and pangolin receptor or human receptor binding of pangolin-CoVs is still Not sure.
In this article, the research team first analyzed the ability of SARS-CoV-2, GX/P2V/2017 and GD/1/2019 to combine pACE2 and hACE2 respectively, and found that SARS-CoV-2 RBD, GX/P2V/2017 RBD and GD/ 1/2019 RBD has similar binding affinity to pACE2 and is about 4 times lower than that of hACE2; the molecular mechanism of the interaction between SARS-CoV-2 RBD and pACE2 is analyzed, and it is found that the binding mode of SARS-CoV-2 RBD to pACE2 is similar to that of hACE2 or hACE2.
Cat ACE2 is similar, but more similar to the binding of hACE2; using homology modeling to further simulate the binding of GX/P2V/2017 RBD and GD/1/2019 RBD to pACE2 and hACE2, respectively, and found that the way they combine pACE2 and hACE2 is similar to that of pACE2 and hACE2.
SARS-CoV-2 RBD is similar and consistent with the affinity data.
SARS-CoV-2 RBD, GX/P2V/2017 RBD and GD/1/2019 RBD are combined with pACE2 and hACE2, respectively.
Comparative article information: [click the link below or read the original text] Wu Lili, Su Jiaqi, Niu Sheng, etc.
New The molecular mechanism of coronavirus binding to pangolin ACE2 receptor.
Chinese Science Bulletin, 2021, 66:73-84, doi:10.
1360/TB-2020-1372
