The crispR-Cas system has great potential for application, ouyang song should reveal the crispR-Cas13d system technical mechanism.

The CRISPR-Cas13 system is capable of targeting editing RNA, known as the "RNA magic scissors."
as scientists study, their family members grow.
, a recent study published in Nature Communications has solved the mystery of crispR-Cas13d, the youngest member of the "RNA Magic Cut" family.
this achievement comes from the team of Ouyang Songying professor at Fujian Normal University.
the team analyzed the high-resolution crystal structure of the Cas13d protein (UrCas13d) and the crRNA binary complex of the tumor gastric bacteria, revealed the key sites of enzyme activity of urCas13d processing precursor crRNA, and identified problems that had not been studied before the interval sequence of crRNA and the mismatch tolerance between the targeted RNA.
", "It's like an aircraft design engineer getting drawings and processes to design and build an airplane, providing a blueprint for repairing or designing a better aircraft."
" Ouyang Song said, this for the further transformation of Cas13d as a nucleic acid editing and detection tools, and to play its application potential in solving human diseases and health issues, provideaforite lying structural basis and theoretical basis.
crispR-Cas system is an immune defense system for pronuclear organisms (bacteria and archaea), which expresses a complex of non-coding RNA (crRNA) and its associated Cas proteins, enabling pronuclear organisms to recognize and "tear up" nucleic acid material (DNA or RNA) that invades the phage, to maintain the "purity" of their genetic material and play an important role in the evolution of the pronuclear organism.
"It is precisely because of this precise targeting of specific nucleic acid sequences that the CRISPR-Cas system has been developed as an efficient gene editing tool for accurate identification and cutting of genetic material DNA or RNA that relies on nucleic acid sequence information."
" Ouyangsong said that the crispR-Cas9 system, in which the reputation is outside, can efficiently edit DNA sequences to correct genetic defects in individual cells, giving hope to many genetic and rare disease treatments, the system has moved from the laboratory research stage to the clinical research stage, has the potential to cure or prevent a variety of human diseases.
in recent years, a class of CRISPR-Cas systems, which can be specifically targeted to detect and edit RNA, have been discovered and reported, named Cas13 (including four family members, a, b, c, and d), derived from the enormous potential of applications in the fields of biological research and medicine.
this RNA-specific editing tool regulates the function of the gene at the RNA level without making permanent changes to the gene itself.
April 2018, a team from the Salk Institute and a team from Arbor Biotechnology in the United States reported on the new Cas protein, named Cas13d, that was screened by bioinformatics and validated by functional experiments.
as the youngest member, Cas13d has functional similarities with other "brothers and sisters", but is more unique.
" the key is size.
" Ouyangsong should be introduced, Cas13d protein sequence length of about 930 amino acids, compared to other Cas13 family members significantly smaller, than Cas9 is 33%, this size makes it easier to package into smaller capacity carriers, such as adenoviruses (AAV) vectors, so in the development of new drugs, new materials and new diagnostic methods have a wide range of potential.
obtaining new "magic scissors" high-resolution crystal structural biology is a branch of molecular biology, biochemistry and biophysics, concerned with the three-dimensional structure of biological macromolecules (such as protein molecules and nucleic acid molecules), obtaining structural information about them, and studying the relationship between the structure and function of biological macromolecules.
"All life activities are achieved through the interaction of various biological macromolecules, and structural biology can explain the conformation and interaction of biological macromolecules, reveal ingenuity mechanisms of life's processes from the atomic level, and make people 'know how to know it'."
" Ouyang Song should tell reporters that the current research scope of structural biology has involved most of the important life activities, molecular biology of each frontier breakthrough is closely related to structural biology.
in this study, Ouyangsong's research team combined structural biology with cutting-edge research topics in life science, and analyzed the high-resolution crystal structure of urCas13d protein and crRNA binary complexes, which can visually see the location of each atom and the details of their interactions. "When an intruder arrives, the CRISPR-Cas system transcribes two 'defensive materials' under the control of the 'commander' (the leading sequence), one of which is the precursor crRNA and the other is the Cas protein we studied, "
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" Ouyangsong should be parable.
this study clearly explains cas13d this "new magic shear", what kind of spatial structure layout is used to process precursor crRNA, and how to combine with mature crRNA to form a functional complex with the ability to identify targetRNA, so as to further study the molecular mechanism of the protein's crRNA-dependent RNase activity.
studies have shown that Cas13d can be used as a powerful RNA editing tool to correct the protein imbalance that causes disease in the cells of Alzheimer's patients.
at the same time, Cas13d can be used as a manipulative RNA binding module, effectively targeting RNA transcriptions in cells, providing a common platform for transcription engineering and disease treatment. "The study has solved the mystery of the CRISPR-Cas13d system, provided a deeper understanding of the characteristics of its technical mechanisms, and helped to make the technology more widely available for the benefit of humanity."

" Ouyang Song should say.
Source: Science Daily.