The design of the overall topic in the field of stroke

called "stroke"
also known as "stroke" and "cerebrovascular accident" (cerebral accident, CVA). It is an acute cerebrovascular disease, a group of diseases that cause brain
tissue damage due to a sudden rupture of blood vessels in the brain or the inament of blood vessels that prevent
blood from flowing into the brain. Stroke has the characteristics of high morbidity, mortality and disability rate, and its symptoms are visual impairment, language disorder, movement disorder, sensory movement disorder, memory disorder, consciousness disorder and so on.
stroke includes ischemic and hemorrhagic stroke. The incidence of ischemic stroke is higher than that of hemorrhagic stroke, accounting for 60% to 70% of the total number of strokes. Ischemic stroke is the general term for necrosis of brain tissue caused by stenosis or closure of the blood supply arteries (cervical arteries and vertebral arteries) of the brain and insufficient blood supply to the brain. China has one of the highest rates of stroke in the world, and epidemiological survey data show that there are about 2×106 new stroke cases in China each year, and there are 10×106 cases of stroke, of which about 85% are ischemic stroke.
With more and more attention to stroke, the hot topics in the field of stroke mainly include: inflammation caused by stroke and its mechanism, anatomy and functional disorders of stroke and its mechanism, treatment of stroke and its mechanism.
stroke project design
1, building animal models (MCAO molding, ICH molding, TBI molding, etc.)
MCAO molding: MCAO is an acronym for middle cerebral artery occlusion, Chinese meaning middle brain artery embolism. The arterial embolism model in the brain does not insert a wire into the artery in the brain, but through the intracranial artery into the skull and into the pre-brain artery, blocking blood supply from the front artery of the brain on the embolism side and blocking the intracranial artery intracranial section that receives the blood supply from the traffic artery.
ICH molding: ICH is an acronym for intracerebral hemorrhage, Chinese meaning cerebral hemorrhage. At present, there are four main ways for the preparation of animal models of cerebral hemorrhage: microsphere sac filling, bacterial collagenase injection, self-hemorrhage injection and spontaneous cerebral hemorrhage model.
TBI: TBI is an acronym for tramatic brain injury, Chinese meaning traumatic brain injury, also known as brain injury or head trauma, specifically brain tissue damage caused by trauma. TBI animal models can be divided into impact brain damage, non-impact acceleration brain damage and explosive shock wave brain damage according to their injury mode and biometric mechanism.
2, model detection (TTC staining, blood flow detection, behavioral testing)
TTC staining: detection of infarction area.
blood flow detection: detection of changes in blood flow.
behavioral score: detecting the effects of stroke on motor ability.
3, drug screening (e.g. preventive drugs, rehabilitative drugs, etc.)
4, molecular mechanism exploration (with the help of AAV vector, the
gene
over-expression, silence)
5, cell therapy (with brain positioning injection, tail intravenous injection
stem cells

/stabilization strain)
6, other esoteric detection (apoptosis index detection, infarction volume detection, cell sample detection, infarction core region, edge region separate detection, gene sequencing, RNA sequencing, electrophysiology)
(the above part of the text originated from the network)