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    Home > Biochemistry News > Biotechnology News > The difference between EPS-G7 and PNP-G7 α-amylase.

    The difference between EPS-G7 and PNP-G7 α-amylase.

    • Last Update: 2020-10-22
    • Source: Internet
    • Author: User
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    : I remember using the PNP-G7 method to measure α-amylase six months ago, and now it's improved to EPS-G7, what's the difference between the two? Which method is better? What about the full English name of the two and the full text of Chinese? I think: EPS-G7 should be 4,6-ethylidene-(G7)-1,4-nitrophenyl-(G1)-α, D-maltoheptaoside
    4,6-ethyl-α, D-Malt 7 glycoside-nitrophenol. Reference from: Shanghai Shennen-Desai
    reagents
    instructions, but I am not sure if it is correct, especially E, P, S three letters represent what? Please also ask the teachers to point!
    : EPS-G7 english full name: 4,6-ethyliden-(G7)-p-nitrophenyl (G1)-α-D-mal-to-topentaoside
    . PNP-G7 English full name: 4,6 -etylidece-(G7)-1-4-nitrophenyl-α-D-mal-topentaoside
    : PNP-G7 method is the IFCC recommended method for determining the vitality of human
    serum
    , urine and body fluids α-amyloid! And it's not interfered with by external enzymes. Serum normal reference value: 0-110 (or 140)! Any urine: 0-640U/L!
    EPS-G7 method (as the owner said Shanghai Shennen-Desai reagent) is to determine the vitality of pancreatic amylase in serum, urine and body fluids, normal serum reference value: 8-53U/L;
    : Both methods actually belong to the malt seven sugar method. The difference is mainly that the substrate used is different, ESP-G7's advantage is that its substrate is the use of ethyl glycoside non-reductive end closed, which can prevent α-glucoside enzyme on the substrate hydrolytic effect, improve the stability of the substrate. EPS-G7:4,6-ethyl-a, D-malt methylucoside-nitrophenol
    PNP-G7: nitrobenzene malt glycosyl..
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