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    Home > Biochemistry News > Microbiology News > The different response of C. D. V9 to different individual intestinal bacteria.

    The different response of C. D. V9 to different individual intestinal bacteria.

    • Last Update: 2020-09-18
    • Source: Internet
    • Author: User
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    .Probiotics in different human responses are different, and the host itself and the environment and many other factors. As a probiotic strain with many excellent benefits, it is important to understand its survival in the intestines and its effect on the structure of the human gut bacteria. Therefore, this experiment explores the survival of Bifidobacteria V9 in 64 volunteers, as well as the effect of differentiation of intestinal bacteria.test designselected a total of 64 qualified volunteers to participate in scientific research, of which 49 testers and 15 validation personnel. It is important to ensure that volunteers were not given antibiotics, had no intestinal disease, and excluded people with BMI of 28 within 3 months of participating in the study. With the consent of the volunteers, each person was given daily milk Bifidobacteria V9 (3.98x
    10
    10
    CFU) to collect stool samples in weeks 2, 4 and 6 (excretation) and to perform a series of tests.Test Results 1. Quantitative determination of C.D. V9 using PMATo ensure the quantitative determination of QPCR of C.D. V9 activity, samples were treated with a stack of nitrogen bromide propylene ingots (PMA). At the beginning of the trial, neither of the samples detected C.D. V9, and during the trial, subjects were divided into three categories (Persisters, Temporary and Non-Persisters) based on the amount and trends of C.I.V. V9 that survived in feces. In the Persisters group (n-23), the average abundance of live milk Bifidobacteria V9 in week 4 was as high as 5x
    10
    6
    CFU (Figure 1) and was washing The de-phased CEDV9 was able to maintain a high level (greater than
    10
    5
    CFU), while there was no significant decrease in live cell abundance in the Persisters group during the exterfusion period. In the Temporary group (n-9), early probiotic intake, the rapid growth of Bifidobacteria V9, the average abundance in week 2 can reach 1.66x10
    5
    CFU, but the abundance during the washesis period decreased rapidly (less than 10
    4
    CFU). For the Non-Persisters group (n-17), live C.D. V9 was not detected before the trial and during the exodus period.. Figure 1: Using PMA to determine the V9 content of mastocytobacteria 2. Response of different individual intestinal bacteria to C. Bifidobacteria V9 Weighted UniFrac distance measurements were performed on samples from three groups at different points in time to reflect changes in each group of intestinal bacteria, and changes in the intestinal bacterigroups in the Persisters group were found to be continuous and significant, but changes in other groups were limited (Figure 2A-C). At the same time, each group is clustered based on NMDS distance (Figure 2D-F) and the changes in the main genus in each group are counted using histograms (Figure 3A). Significant changes were found in the abundance of seven genus, including Bifidobacteria, Shigella, Echella, Botox, salmonella,
    Hydrogenoanaerobacterium
    and Campylobacterium in the Persisters group (Figure 3B).
    . Figure 2: Reactions of different intestinal bacteria to C.D. V9Figure 3: Changes in the genus of three groups of intestinal bacteri 3. Comparative analysis of the structure and characteristics of three groups of individual intestinal virlogroups Persistence of probiotic survival in the intestines and many factors For the analysis of the composition and characteristics of the gut bacterigroups in three groups of samples (e.g. age, sex and BMI), the Shannon index and the super-1 index showed significant differences in alpha diversity between the Persisters and Non-Persisters groups, which were significantly lower in alpha diversity than in the Non-Persisters group (Figure 4A, B). The t-SNE analysis structure showed that the gut bacteri groups in the Persisters and Non-Persisters groups were not highly different (Figure 4C).
    . Figure 4: Comparative analysis of the structure and characteristics of three groups of gut microorganisms 4." The Anti-Plant Index (AEI) predicts the stability of C.D. V9the relative abundance distribution of all identified genus in each group of samples (Figure 5A), and by determining the genus of differential distribution between the Persisters and Non-Persisters groups, a total of 12 genus were found to be associated with the stability of Bifidobacteria V9, of which Bifidobacteria,
    Flavonifractor, Olella

    Acetanaerobacterium, Asaccharobacter, Lachnoclostridium, Blautia
    and clostridium were negatively correborated with probiotic stability, while anaerobic bacteria, abicobacteria and
    Erysipelatoclostridium
    were positively correaded with probiotic stability (Figure 5B).
    . Figure 5: Differences in the distribution of gut microorganisms between the Persister and Non-Persister groupsBased on the relative abundance of the 12 genus above, the "anti-plant index" (AEI) was calculated:based on the assessment of the stability of CFO V9 by these biomarkers, significant differences in AEI values were found between the three groups (P 0.001; Figure 6A). The performance of the AEI exponential model in the tester (n-49) was evaluated using the subject's working characteristic curve (ROC). The Youden index is determined from the ROC curve, where the AEI threshold is 0.87, and when the AEI index reaches this threshold, the stability of Bifidobacteria V9 is limited. The area under the ROC curve is 85.45% (Figure 6B), indicating that the AEI index can evaluate the stability of CLO V9 with high precision.. 5. Testing the "anti-plant index" (AEI In order to verify the performance of the microbial model, 15 validators participated in the test, each subject took CFC V9, collected stool samples at the same point in time as the tester to test, and calculated AEI, found that the probiotic stability of the validator AEI index accuracy rate reached 85.71%, to meet the high-precision assessment (Figure 6C).
    . Figure 6: Performance and testing of the "anti-plant index Test Conclusions The response of Clocal Bifidobacteria V9 in the intestines of different individuals is different, in some people can be very good implantation, with strong stability, while in another part of the human body can only temporarily stay or can not be planted. The implantation of Bifidobacteria V9 can also change the structure of the human gut bacteria and alpha diversity. At the same time, 12 genus were found to be related to the planting ability and stability of C.C. V9, and the biological model "Anti-Tymogenic Index" (AEI) was designed to evaluate the intestinal stability of C.D. V9, and its accuracy was found to be as high as 85.71%, which can effectively assess the stability of C.C. Bifidobacteria V9 in the body.
    .
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