The function of lincRNA in beautiful nematodes.

The Research Team on Non-Coding RNA Function and Mechanism at the School of Life Sciences of the Chinese University of Science and Technology recently published an article entitled EvaluationSystematic of C.elegans LincRNAs with CRISPR knockout mutants in the international journal Genomic Biology, reporting on the latest laboratory research results.
the lab systematically studied the function of lincRNA in the software nematodes by optimizing gene editing techniques to remove the long-chain non-coding RNA (lincRNA) between 155 genes in the software nematode (a total of 170 of the total total of 170 lincRNA known to the software nematodes).
this is the first study to use gene editing technology in multicellular animals, knock out a species of long non-coding RNA at the genome level, and systematically analyze its physiological function and mechanism in animals.
their screening of 155 lincRNA knockout mutants on six phenotypes found that 23 lincRNA mutants had varying degrees of defects in one or two of the physiological phenotypes.
by analyzing the transcriptomic sequencing of different developmental periods of the beautiful nematode, the co-expression of lincRNA and mRNA was studied, and a network of co-expression and regulation of lincRNA and microRNA was established.
to explore the regulation of lincRNA expression at different stages of the development of transcription factors in the online development of the functionality of the functionalization of the 23 lincRNAs by analyzing the analysis of nearly 300 transcription factors in different developmental periods of the beauty nematodes, and then study the mechanism of these 23 lincRNA seowere physiological regulation functions.
the study systematically explores the physiological function of lincRNA in multicellular animals, broadening the field of lincRNA research to a certain extent.
also the study's lincRNA knockout nematode strains provide strong support for further research into the role of long-chain non-coding RNA in aging and disease. The co-authors of the
article are Dr. Wei Shuai, postdoctoral student Chen Wei, and recently ph.D. graduate International Student Emmanuel Enoch Dzakah, and the communications author is Professor Shan Ge of the University of Science and Technology of China.
the research is supported by the Ministry of Science and Technology, the State Fund Committee and the Chinese Academy of Sciences, "the biological basis of aging and intervention strategy" pilot science and technology special (cultivation).
Source: China University of Science and Technology.