 Home > Biochemistry News > Microbiology News > The Generation and Selection of Single-Domain, V Region Libraries from Nurse Sharks

The Generation and Selection of Single-Domain, V Region Libraries from Nurse Sharks

 Last Update: 2021-01-26
 Source: Internet
 Author: User

Tags

immune system smoothie

single chain antibody

Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com

The cartilaginous fish (sharks, skates, and rays) are the oldest phylogenetic group in which a human-type adaptive immune system and immunoglobulins (Igs) have been found. In addition to their conventional (heavy-light chain heterodimeric) isotypes, IgM and IgW, sharks produce the novel isotype, IgNAR, a heavy chain homodimer that does not associate with light chains. Instead, its variable (V) regions act as independent, soluble units in order to bind antigen. In this chapter, we detail our immunization protocol in order to raise a humoral IgNAR response in the nurse shark (
Ginglymostoma cirratum
) and the subsequent cloning of the single-domain V regions from this isotype in order to select antigen-specific binders by phage display.

This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

Related Products

Fecal coliform positive control samples (multi-tube fermentation) BNCC359094 BNCC

CAS No.:

$220-280/MT EXW

Standard quality control sample of fecal coliform (multi-tube fermentation method) BNCC359289 BNCC

CAS No.:

$220-280/MT EXW

Water Quality Total Coliform Standard Quality Control Sample (Paper Rapid Method) BNCC360407 BNCC

CAS No.:

$220-280/MT EXW

Hot Tags

united states jobs(223)

jobs united states(236)

1 year treasury(277)

1 year subscription(296)

announcements today(81)

80 20 company(94)

united states logo(227)

single use technology(195)

healthy food name(42)

ups united states(221)

Hot Articles

The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.