The genome map of black otters is revealed and the quality of the black otter varieties is improved.

Huang Yongping, Zhan Shuai and Wang Sibao of the Institute of Plant Physiology and Ecology of the Chinese Academy of Sciences, in collaboration with Zhang Jibin team of Huazhong Agricultural University, Zhang Zhijian team of Zhejiang University and Yang Hong team of Huazhong Normal University, studied and revealed the high-quality genome map of The Black Water (BSF), and developed an efficient genome editing method based on CRISPR/Cas9, which provides valuable genomic and technical resources for optimizing The industrialization of BSF.
the results of the study were published online in Cell Research.
the global population increase means an increase in the amount of organic waste.
organic waste generated in urban environments is mainly treated by incineration, landfilling and composting.
these treatments not only result in the waste of resources, but also cause secondary pollution to the environment.
black water can efficiently utilize many types of organic waste and convert it into valuable products.
such as black-water moth protein is a high-quality animal feed protein source, insect fat can be used in industry and living raw materials, insect waste can be used as a high-quality fertilizer.
BSF's mass-scale production technology has matured and is one of the most promising insect species in the world.
more importantly, bSF is by far the few insect species in the world that has been approved for use as an ingredient in aquaculture and poultry feed.
scientific studies show that the use of BSF to dispose of organic waste consumes very little energy.
the amount of organic waste treated by BSF, whether in insects or in insect dung, the amount of pathogenic bacteria and antibiotics has decreased significantly.
, bSF's practical and unique biological characteristics, combined with parsed genomic data and the established gene-editing technology platform, are becoming model organisms that transform organic waste and are rapidly being rolled out in the insect farming industry.
in this study, a team of scientists has constructed a high-quality genome map of black otters, which will help domestic and foreign counterparts to reveal the molecular mechanism of black otters adapting to harsh environments, defending against pathogenic microorganisms, removing odors from living environments, and degrading antibiotics.
on the other hand, the team developed an efficient genome editing method based on CRISPR/Cas9 for the genome editing of black otters, and used this method to construct the black otter mutant phenotype, which is loss of flight and increased body size, both of which contribute to the production performance and ecological safety of black otters.
therefore, the study provides new breeding methods and important basic data for promoting the industrialization of black otters.
research reveals the genetic basis of the unique biological characteristics of black otters and achieves product improvement On November 26th, the international academic journal Cell Research published online the team of "Genomic and The
the study revealed the genetic basis of the unique biological characteristics of black otters by integrating genomes, transcription groups, macro genomes and other multi-group methods, and realized the initial improvement of the black otter line by means of gene editing.
with the increase of population and the development of urbanization, urban household waste and other pollution problems are becoming more and more serious.
traditional waste disposal methods (e.g. landfill, incineration, etc.) can not meet the requirements of national ecological civilization construction planning and sustainable urban environment development due to land use and secondary pollution.
excreta produced in rural poultry and livestock rearing also needs better treatment.
new harmless and sustainable waste disposal methods need to be developed and utilized.
Hermetia illucens is a rotting bi-winged hydroponic insect that can survive in a dirty environment and take organic waste such as faeces and convert it into a high-protein stored in itself, widely used in high-value animal feed.
therefore have great potential for the resource-based use of organic waste.
but for a long time, due to the lack of genomic resources and genetic operation methods, the functional research and genetic improvement of the molecular level of black otters have been severely restricted.
researchers constructed a high-quality genome of 1.1Gb of black otters through high-throughput sequencing and bioinformatics analysis, and predicted 16,700 black hydroprotein encoded genes with a genome coverage of nearly 100%.
the genome of the black otter is much larger than that of the major bivalve flies, and there are a large number of duplicate fragments and genome replication.
comparative genomic analysis showed that the detoxifying genes, olfactory receptors, immune pathways and other gene families of black otters expanded significantly.
especially the immune pathway identification and effect module, and at the same time in the black otter and home fly genome large replication, suggesting that insects' adaptation to dirty environment may be achieved through the replication of the immune identification and effect module.
researchers further explore the changes in the gene expression and intestinal microbiome of black otters after taking different feces and kitchen waste, and found that black otters respond to different types of organic waste with the same gene, including a large number of immune, digestive-related genes and species-specific genes, while the diversity of intestinal flora is greater by the influence of different types of organic waste, but the thick-walled bacteria have always occupied an advantage.
researchers also established for the first time a black otter gene editing platform, through CRISPR/Cas9 technology successfully knocked out the black otter hysteresis-related gene Ptth and wing development regulatory gene Vg.
, the development of ptth mutant black water slugs stagnated in the larvae period of more than 90 days, can achieve continuous feeding during the larval period, significantly improve the utilization efficiency of black otters; The research and application of
resource insects is an important research field in insect science.
Molecular Plant Excellence Center has a number of teams engaged in the study of resource insects such as silkworms and black otters, and is committed to revealing the molecular mechanism of resource insect importance by means of gene editing, synthetic biology, and realizing the genetic improvement and industrialization of resource insects.
the research was completed by a number of scientific research units and a number of research groups, and the research results laid an important foundation for the improvement and industrialization of the black otter system. Zhang Jibin, a professor at Huazhong Agricultural University in
, contributed important water products and related materials, and Zhan Shuai and Wang Sibao each played an important role.
Molecular Plant Excellence Center graduate students Fang Gangqi, Ko Zongqing and Huazhong Agricultural University Ph.D. Tsai Wei as co-authors of the thesis, Huang Yongping and Zhan Shuai, Zhang Jibin and Jeffery K. Tomberlin of Texas Agricultural University in the United States as co-authors; Yang Hong, Zhang Zhijian and other guidance of part of the work.
the study was funded by projects from the Chinese Academy of Sciences and the Ministry of Science and Technology.
Synthesis of this article: Huang Xin, China Science Journal, Center for Excellence in Molecular Plant Science Innovation/ Institute of Plant Physiology and Ecology Source: Huang Xin, China Science Journal, Center for Excellence in Molecular Plant Science/Institute of Plant Physiology and Ecology.