The Guangzhou Institute of Biology of the Chinese Academy of Sciences has developed a 5G version of somatic cell reprogramming technology

team led by Yan Tingqing, a researcher at the Guangzhou Institute of Biomedicine and Health of the Chinese Academy of Sciences, developed a new method of high-efficiency reprogramming using 7 factors instead of traditional 4 factors (OKSM). This method is like upgrading mobile communication signal from "4G" to "5G", providing high-quality cell sources and new cell models for the study of regenerative medicine and the mechanism of inducing erythmatic stem cells. The study was published online June 18 in Cell-Report.
Since 2006, when Professor Yamatsahma Yamano of Kyoto University in Japan reported that four transcription factors, Oct4/Sox2/Klf4/c-Myc, can reprogram somatic cells into erypotent stem cells, the technology has attracted attention for creatively avoiding issues of immune rejection and ethical controversy, opening a new chapter in cell biology. In recent years, the research shows that induced erythnic stem cells have broad application prospects in cell therapy, tissue organ repair, disease model, drug screening, precision medicine and other fields.
scientists have developed different reprogramming systems to understand the secrets of open cell "changes" and advance clinical applications. It turns out that the urinal cells fold genomic DNA and histones at different levels into chromatin, and the chromatin's closing or opening state is closely related to the reading of precise information related to the fate of cells. The team found that changes in chromatin state during soy cell reprogramming followed certain rules.
It is in accordance with the law of chromatin dynamic changes in the reprogramming process, from the point of view of on and off, combined with gene expression spectrum analysis, the scientific research team led by Yu Tingqing developed a new high-efficiency reprogramming factor mixture consisting of 7 factors (7F), which can quickly reprogram mouse fibroblasts into iPS cells. This mixture consists of five transcription factors Sall4, Esrrb, Nanog, Glsi1, Jdp2, and two oscic modification factors, Kdm2b and Mkk6.
pointed out that the system can be used to increase the reprogramming efficiency of traditional OKSM from less than 0.1% to about 10%, and at the rate, only need to reprogram for 4 days to obtain iPS cells capable of ingling mice and reproductive transmission mice. If the reprogramming process is likened to information communication, the birth of 7F is undoubtedly the original "4G" to the "5G" fast track. Unlike the Yamanaka factor, 7F selects a specific "channel" that pushes so many cells to the end of the iPSC, where the reprogramming factors work together to regulate the opening and closing of the corresponding bits.
this study reveals that the combination of reprogramming factors designed to follow the laws of chromatin dynamic change plays an important role in determining the characteristics of iPSCs, which facilitates the rapid access to high-quality iPSCs and provides more options for further revealing reprogramming mechanisms. At the same time, rapid short-term access to high-quality iPSCs can shorten the cell therapy process and accelerate the progress of stem cells and regenerative medicine to clinical.
addition, this factor screening concept helps scientists design transcription factor combinations to alter chromatin structures, combine small molecular compounds, and make it easier to manipulate cell fate decisions.