The key genes that determine the stress tolerance of polyspherealgae.

Blue bacteria is a kind of ancient photosynthesis microorganism, in order to cope with the changes of light, temperature and other environmental conditions, gradually evolved a set of efficient environmental stress tolerance mechanism.
polysphere UTEX 2973 is a newly discovered algae strain that is well-tolerated to high temperatures and strong light conditions and can grow rapidly in light conditions up to 42 degrees, 1500 m-2 s-1, for only 1.5 hours.
under the same conditions, most blue bacteria, including its near-species polysphere PCC7942, cannot grow.
interesting, the genome sequence consistency of uTEX2973 and polysphere PCC7942 is as high as 99.8%, and they have only 24 differential protein-coding genes, but have significantly different environmental tolerances.
, therefore, these differential genes are likely to be the direct cause of the difference in the two clusters of polysphere stress tolerance phenotypes.
In order to identify the key genes that determine the tolerance of polysphere stress tolerance, the Microbial Metabolism Engineering Research Group led by Lu Xuefeng, a researcher at the Qingdao Institute of Bioenergy and Processing of the Chinese Academy of Sciences, adopted a "gene complementary" strategy: treating polysphere PCC7942 as a gene-defective mutant strain, and transforming polysphere CHP7942 into a gene fragment of polysphere UTEX2973, screening the mutant strains with high temperature and high light tolerance.
found that all high temperature highlight-tolerant polysphere CHP7942 mutant strains had a c252Y (trypine-to-luinin) point mutation in 252 amino acids in its FoF1 ATP synthase ɑ at at at-taarine.
and the saturation mutation at this site was found to have the mutation of cysteine (Cysteine, C) into any conjugateamino acid (phenylalanine, lianine, histanine, tryptophan) that enabled polysphere PCC7942 to achieve high-temperature highlight tolerance.
through the system's biochemical, physiological and metabolic level sourcing, the C252Y point mutation caused a significant increase in foF1 ATP syntin level ɑ FoF1 ATP synthase level and FoF1 ATP synthase activity, increased the intracellular ATP level;
the study identified key genes that determine the environmental stress tolerance of fast-growing polyopheria, providing an important target for metabolic engineering to modify photosynthesis bioenvironment resistance (Appl Environ Microbiol, 2018).
in response to the problem of ambiguous transcription regulation mechanism under the stress conditions of polysphere UTEX2973, the research team, in collaboration with Wolfgang Hess, a professor at the University of Freiburg in Germany, conducted a differential transcription almandomics study based on dRNA-seq, analyzed the effects of different stress conditions on the transcription and physiological metabolism of the polysphere UTEX2973 gene, and found that the Sye_sRNA1 and the Sye_sRNA3 of the large number of macro-theomic stoic. In addition,
the study accurately identified 4,808 transcription starting sites in the whole genome range of UTEX2973, laying the foundation for subsequent transcription regulation and research on metabolic engineering modification (Biotechnol Biofuels, 2018).
research has been supported by the National Natural Science Foundation of China's Distinguished Youth Fund, the Chinese Academy of Sciences key deployment projects, Shandong Province's major basic research and so on.
related results published: (1) Lou W, Tan X, Song K, Zhang S, Luan G, Li C, Lu X. (2018). Single SNP in ATP synthase gene tyau ly ei cyntaar of Synechococcus elongatus PCC 7942.Appl Environ Microbiol doi: 10.1128/aem.01222-18. (2) Tan X, Hou S, Song K, Georg J, Kl? hn S, Lu X, Hess WR. (2018). The primary llome of the fast-growing cyanotruum Synechococcus elongatus UTEX 2973.Biotechnol Biofuels 11:218.