The Korea-Fangpu research team made progress in the study of plant chromosomal histone phosphate

phosphorylation modification is associated with the establishment and maintenance of silk grain function (Dong and Han. 2012). Since 2010, the laboratory has been engaged in phosphorylation modification and chromosomal orientation and separation of corn, wheat H2A and H3. Because of the complexity and particularity of plant chromosomes, we have been getting some different results from yeast and humans. The positioning and cell cycle changes of the H2A phosphatized kinase Bub1, combined with RNAi and H2A phosphatization signal changes, found in the corn's special tiny chromosomes, minus division mutants, the compound is not related to the orientation of the subtracting I chromosome with silk grains, and the results associated with the activity of the silk particles have been published in early 2017 in New Phytologist (Su Phytologist. 2017）。 We have been trying to find the relationship between H3T3 phosphatization and chromosomal dynamic changes, the relationship between silk particle activity and its mechanism of change. After years of efforts, the H3T3 phosphatized kinase Haspin antibody was obtained and the cytological observation of the corn minus division mutant was carried out in detail.
results of antibody immunofluorescence of H3T3 phosphorylation antibody showed that there were early and mid-stage chromosomes with silk division, and H3T3 phosphorylation occurred between sister chromosome monomers. Dynamic changes in cell cycle H3T3 phosphate and the results of corn de-deflation division mutants showed that H3T3 phosphate was associated with cohesion. It is interesting to see that the inactive silk particle site also has early signals of H3T3 phosphate (Figure a). This result is completely different from H2A phosphate and H3Ser10 phosphate only occur in the functional grain region. H3T3 phosphatization signals appear only in inner centromere as chromosomal contraction enters the mid-term ready to complete the correct orientation and separation of chromosomes (Figure b). The H3 nucleosome part of the plant's silk grain region is replaced by the CNH3 nucleosome, but on the early and early mid-chromosomes in the silky region, the CNH3 nucleosome in the silk grain region is mixed with the H3 nucleosome, and there is no specialization of the region or location. However, when the chromosome enters the mid-term ready to begin to complete the orientation of the two poles, the inner centromere is the region formed entirely of the H3T3 nucleosome, while the outer side is dominated by the distribution of the CNH3 nucleosomes. May be closely related to spindle's correct connection and functional particle formation. In-depth study of moving granules and their regulatory processes is under way.
the preliminary findings were published in the journal
on October 16, 2017 (DOI: 10.1111/tpj.13748), with Liu Yang, Su Handong and Liu Yalin, phD students from the Korea-Pamp Research Group, as the first authors of the paper. This research work is supported by the National Natural Science Foundation of China. (Source: Science.com)