The loop arrangement may affect the physiological function of G4-DNA.

Recently, Li Can, a member of the National Key Laboratory of Catalytic Sciences of the Dalian Institute of Chemical Physics of the Chinese Academy of Sciences, and Cheng Mingpan, a doctoral student, made progress in the study of the structural properties and catalytic function of G-tetra-chain nucleic acid (G4-DNA), and found that the arrangement and combination of the loop regional sequence of G4-DNA has an important effect on the secondary structure and thermal stability of G4-DNA that is formed by the folding of the DNA sequence of the bird's radon (guanine, G).
related research is published in nucleic acid research. The structure and properties of G4-DNA at the end of human telomeres
is a cutting-edge topic in the study of nucleic acids.
Li Can's research team conducted earlier research, especially the exploration of the enzyme catalysis function of G4-DNA, in the hope that DNA catalytic research can contribute to human health research.
earlier research by the team found that human telomere G4-DNA can catalyze asymmetric Diels-Alder reactions (Ange.Chem.Int.Ed.) and Friedel-Crafts reactions (Chem.Commun.), extending a single G4-DNA to multiple G4-DNAs that significantly improves the selective stereoscopic and catalytic activity of the reaction (ChemChemBio; Chem.Commun.).
later, the team developed a highly active high-activity, high-dimensional selective DNA enzyme (Chem.Sci.) using a potential anti-cancer drug that targets g4-DNA as a catalyst, and reported the first case of asymmetrical oxidation reactions (Chem.Commun.) from DNA catalysis.
recently, the team developed a fluorescence-annihilation method for the study of the asymmetric catalytic reaction mechanism of DNA (ChemBioChem; Biochimie).
these efforts confirm that G4-DNA has an enzyme catalytic function in response to some physiological conditions.
Early studies based on G4-DNA sequences at the end of human telomeres found that by changing the relative position of the loop, G4-DNA was transformed from a hybrid structure into a parallel structure, significantly increasing the catalytic activity of the G4-DNA and hemoglobin-like hydrogen peroxide enzyme (Biochim.Biophys.Acta).
found in this work, the dependence of the G4-DNA folding structure on the loop arrangement is a universal law, changing the relative arrangement position of the loop sequence, G4-DNA may collapse into parallel, hybrid or anti-parallel conformation, and affect the melting point temperature.
statistical law shows that when the long loop is in the middle position, the sequence of this class is more likely to form a high-stability non-parallel structure, and when the short loop is in the middle position, the sequence of this class can form a parallel structure with relatively low stability.
bioinformatics analysis found that most of the 21 sequences studied in the study were present in the chromosomes of a variety of organisms, leading to the inference that the loop arrangement might affect the physiological function of G4-DNA.
the research work was funded by the National Natural Science Foundation of China and the Natural Science Foundation of Liaoning Province.
Associate Professor Zhou Jun of Nanjing University and Jean-Louis Mergny, Professor of the French National Institute of Health and Health (INSERM), participated in the collaborative study.
the research group of the special special area of thermochemical innovation of Dalian Chemical Institute and the research group of nuclear magnetic technology provided support in the experiment of nuclear acid melting point and the nuclear acid nuclear magnetic resonance experiment of the isometric temperature drop quantitative detection nuclear acid, respectively.
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