The method of executing experimental animals

"experimental animal there are many methods of death, according to the animal experimental purposes, experimental animal breeds (species), as well as the need to collect specimens and other factors, choose different methods of death. Either way, the principle of euthanasia should be followed.

euthanasia refers to the short-term and painless death of experimental animals without affecting the results of animal experiments.

The death of experimental animals should be noted, first of all to ensure the safety of laboratory personnel, the second is to confirm that the experimental animals have died, through breathing, heartbeat, pupils, nerve reflexes and other signs of observation, to make a comprehensive judgment of death, and then pay attention to environmental protection, to avoid pollution of the environment, but also to properly handle the body.

1, cervical dislocated death method

this method is the experimental animal's cervical vertebral dislocation, broken spinal cord to death, for large, mouse the most commonly used method of death. During the operation, the experimenter grabbed the root of the sage with his right hand and lifted it, placed it on the cage cover or other rough surface, pressed the mouse's head and neck down with his left thumb and index finger, and forced the right hand to hold the root of the sage to pull back, resulting in dislocated cervical vertebrae, broken spinal cord and brain dry, and the experimental animal died immediately.

, severed head and

applied to smaller experimental animals such as rats. During the operation, the experimenter used his left hand to hold the back of the experimental animal, the thumb clamped the right armpit of the experimental animal, the index finger and middle finger clamped to the left forehead, and the right hand cut the mouse's head vertically in the mouse neck with scissors, so that the experimental animal died from a severed cerebrospinal cord and a large amount of bleeding.

, hit the skull to death method

mainly used for guinea pigs and rabbits to be executed. During operation, the tail of the experimental animal is grabbed and lifted, and hard objects such as a wooden hammer are used to hit the experimental animal's head hard, damaging the brain center, spasms and death of the experimental animal.

, blood-letting method of death

this method applies to a variety of experimental animals. The specific method is to cut or cut the artery, neck artery, abdominal aorta of the experimental animal, pierce the heart of the experimental animal to shed blood, resulting in acute haemorrhage, shock, death.

dogs, monkeys and other large animals should be in a mild anaesthetic state, in the triangular cross-section, the arteries, veins are all exposed and cut off, so that blood flow. When operating with tap water to continuously flush the inflow and blood, not only to keep the blood flowing unobstructed, but also to keep the operator's station clean, so that the experimental animals acute haemorrhage death.

, air embolism to be executed

to kill rabbits, cats, dogs commonly used this method. Inject a certain amount of air into the veins of experimental animals to form pulmonary or coronary artery air embolism, or lead to filled the heart cavity with bubbles, the heart contraction bubbles become smaller, the heart clevenous bubbles become large, thus affecting the amount of refringing blood and heart output, causing circulation disorders, shock, death. Air embolism injection of air, cats and rabbits for 20 to 50 ml, dogs for 90 to 160 ml.

, over-narcotics method of

is used to kill guinea pigs and rabbits. Rapid overdoses of non-volatile narcotic drugs (30 times the amount given during deep anesthesia) or the inhalation of excess ether by animals cause excessive inhibition of the central nervous system in experimental animals, leading to death.

, gas death method, so that

animals inhale a large number of CO2 and other gases and poisoning death.