The method of inoculation of spiral plate method.

1, the general requirement is to use a spiral inoculation instrument to inoculation samples on the tablet.
the samples, the bactericides were distributed on the spiral trajectory, and the distribution became more and more thin with the increase in radius.
a special counting grid, the number of microorganisms in the sample can be obtained by counting the bacteriocemics on the flat dish from the outside of the plate to the center.
2, the experimental step to prepare a good dilution of the sample dilution, in the selected mode inoculated in the test plate, each dilution inoculated two plates, inoculated before and after each sample according to the instrument set procedures for the spiral inoculation instrument cleaning and disinfection.
the same vaccination pattern as a blank control.
the plate is cultured at the culture time and temperature specified in the standard method, the number of flat bacterios per plate is counted and recorded.
3, the total number of places calculated and recorded the number of places on the tablet in line with the fall counting meter specified counting range for the appropriate range.
If the number of four flat-panel bacterios with two dilutions is within the appropriate range, the average number of flat-panel sterilises is used as the number of steriles per gram (ml) of the sample, and if the number of two flat-panel dills with only one dilution is within the appropriate range, the average number of flat-panel sterilises is used as the number of bacteriograms (ml) of the sample.
When the number of flat bacterios with low dilution is less than the lower limit of the appropriate range, the average of the number of flat bacterios per gram (ml) of sample is calculated.
the number with an asterisk , indicating that the number is derived from a tablet estimate where the number of bacterios is outside the range of the count.
When the number of drops on all plates exceeds the upper limit of the appropriate range, the average of the two flat dices with high dilution is calculated as the number of drops per gram (ml) sample, with an asterisk on that number.
If all diluted plates do not have bacterios, add an asterisk to this number by multiplying by less than 1 times the dilution multiplier and inoculation volume as the number of drops per gram (ml) of the sample.
, only when the number of drops per gram (ml) of sample can be calculated, two valid digits can be set, and the third digit is recorded by rounding.
can also record the number of places in the sample as an exponential form of 10.
4, the expression of the results according to the F.3 archive calculated the number of bacteria per gram (ml) sample, solid samples in CFU/g, liquid samples in CFU/mL units.
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