The module particle assembly system for the study of gene expression of yellow monocytobacteria in rice came out

construction and application ofmodule plasm matter assembly system
Chen Gongyou of Shanghai Jiaobi University School of Agriculture and Biology team developed a module plasmbug assembly system, which solves the bottleneck of low-copy vector DNA genetic operation efficiency applicable to rice jaundial monocytobacteria, transfers functional DNA module to low-copy skeleton vector through high-copy genetic background, for gene protein expression, promoter activity, functional complementarity of mutants, and pathogenic pathogenic pathology. The findings were published online online in Molecular Plant Pathology.
plant pathogen jaundial monocytobacteria (spp.) can cause diseases in more than 400 plants and cause serious economic losses. On rice, by the rice-yellow monocytobacteria (X. oryzae) 2 pathogenic variants of rice white leaf disease bacteria (X. oryzae pv. oryzae, Xoo) and stripes (X. oryzae pv. Oryzicola, Xoc) caused by white leaf disease and strip disease is a very important two bacterial diseases in rice production, seriously threatening the safe production of rice in China. The interoperability of Xoo and Xoc with rice has been used as a model system for pathogenic bacterial pathogenic mechanisms and host tissue specific studies.
Xoo and Xoc strains have more restrictive modification systems that allow only vectors with a wide host range to replicate and stabilize their presence within their cells, which are generally low-copy, making DNA genetic operation more difficult. Therefore, it is of great significance to break through this technical bottleneck and establish an efficient carrier system for gene expression research.
To this end, the researchers designed a modular prosabolic assembly system, using high-copy entry vectors and target vectors, combined with synthetic biology methods, the core components of gene expression into modules, through assembly, fusion elements in the genetic operation of high-copy primer into low-copy "skeleton" carrier, the yield of positive cloning increased by 20 times. The researchers demonstrated that this modular particle system can be effectively applied to transcriptional expression and protein expression analysis of Xoo and Xoc toxicity-related genes in in-body, host rice and non-host tobacco. In addition, the introduction of different terminating sub-elements in the skeleton carrier can give different expression levels of the target protein, according to different experimental needs to customize the expression level of the target gene, applied to the functional complementary study of gene mutants.
experts said that the carrier system is simple, efficient, multi-functional, and low economic cost, but also broke through the limitations of the original system, can be in living plant tissue for quantitative determination of gene expression. Because of its wide host characteristics, it is also suitable for other plant pathogens, jaundial monocytobacteria. (Source: Huang Xin, China Science Journal)
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