The most commonly used tool enzyme in DNA recombination techniques.

the
nucleic acid
inchease
identifies
DNA
specific sequences, cutting off DNA strandsDNA polymerase I.
or its large fragments (Klenow) (1) gap translation to make a marker DNA probe (2) synthetic
cDNA
second chain (3) to fill the double-stranded DNA 3' concave end (4) DNA sequence analysis
heat-resistant DNA polymerase (Taq) DNA polymerases, etc.) polymerase chain reactions (
PCR
) DNA connective enzymes
connecting two DNA molecules or fragments do
nucleotides
kinases
Catalytic polynucleotide 5' hydroxy-end
phosphate
, preparation end marker probe end
transserase
add homogeneity polymer tail at the 3' end.. SI nuclease, mung bean nuclease
degrades single-stranded DNA or RNA, turning the protruding end of double-stranded DNA into a flat-end DNA endase I.
degradation of DNA, the production of random incisions on double-stranded DNA RNAaseA
de-dissolution RNA phosphatase
removal of nucleic acid end phosphate basenucleasenucleic acid exoceps
): from one end of the nucleic acid, one after another to hydrolyzed nucleotides;nucleic acid intra-cut enzyme
( endonuclease ): is hydrolyzed from the middle of the nucleic acid chain 3', 5' phosphate phosphate deester bond, the nucleic acid chain is cut off.. DNA restrictive endoenzymes
sticky ends are paired and recombined sexual cleavage enzymes
and there are some different sources of restriction enzymes that identify the same nucleotide target sequence, which is called isolytic enzymes. The same cutting is produced with the cleavage enzyme, forming the same end.are similar to
ases, which come from different sources and identify different sequences of target words, but produce the same sticky ends. Dna fragments produced by isoenzymes can be connected to each other through complementary effects between their viscous ends..