The Plant Journal Professor Nan Yao's team from Sun Yat-sen University reveals the molecular mechanism of ceramide regulating plant resistance

As the main component of the cell membrane system, sphingolipids participate in the regulation of many cell biological processes
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Ceramide is the core molecule of sphingolipid metabolism pathway and plays a key role in the process of sphingolipid-mediated signal transduction
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However, the key target protein downstream of ceramide is still unclear
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Recently, the team of Professor Nan Yao from the School of Life Sciences of Sun Yat-sen University published a research paper entitled Ceramides regulate defense response by binding to RbohD in Arabidopsis online in The Plant Journal, revealing the mechanism of ceramides regulating defense response by binding to RbohD in Arabidopsis
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The study found that mutating the ceramidase gene ACER in the Arabidopsis ceramide kinase mutant acd5 background, the obtained double mutant acer acd5 showed a specific high accumulation of ceramide, while other sphingolipid components were similar to wild-type plants.
Compared with no significant change
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At the same time, the salicylic acid (SA) and reactive oxygen species (ROS) signals in acer acd5 are continuously activated
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By observing the location of ROS production in acer acd5 cells, it is found that the plasma membrane is the main location of ROS production
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By expressing RbohD protein in vitro and performing protein-lipid binding experiments, it was found that ceramide can directly bind to RbohD
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In vivo and in vitro enzyme activity experiments proved that ceramide can promote the activity of RbohD, indicating that ceramide mediates the regulation of plant resistance through RbohD
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Figure 1.
Ceramide binds to RbohD and promotes its enzyme activity.
This study proved for the first time that ceramide can directly bind to RbohD and promote its activity.
It clarified the downstream target protein of ceramide and provided a new idea for elucidating the biological functions of plant sphingolipids
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The research was funded by the National Natural Science Foundation of China and the Natural Science Foundation of Guangdong Province
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