The PRC2-mediated H3K27me3 modification can effectively buffer ABA-induced plant apoptosis.

On October 11th, the Journal of The Plant Journal published an online research paper entitled Poly recomb recoms hot hot 2 attenuates ABA-induced senescence in Arabidopsis, Center for Excellence in Molecular Plant Sciences and The Institute of Plant Physiology and Ecology of the Chinese Academy of Sciences.
the study revealed that PRC2-mediated H3K27me3 modifications effectively cushion ABA-induced plant apoptosis.
plant hormone acid (ABScisic Acid) plays a key role in increasing plant tolerance to abiotic stress, such as drought, and recent studies have shown that ABA can increase plant tolerance by inducing leaf apoptosis to promote plant re-use.
but excessive apoptosis can affect plant survival, and several pathways that limit transcription levels of ABA response have been revealed.
the early study of the research team found that the development of the important epigenetic complex multicomb family protein (Polycomb repressive complex 2, PRC2) mediated H3K27me3-bit a large number of rich ABA response components (ABA response element, ABRE) (A), so how the ABA response is regulated at the epithelial level? By treating core PRC2 component mutants CLF and SWN with an external ABA, the researchers found that the twin mutants were more sensitive to ABA processing (B).
analysis of mutation transcription group changes found that a large number of ABA-induced expression genes increased expression (C) in double mutants and were primarily involved in the apoptosis process (D).
, in dual mutants, ABA's induced expression of apoptotic-related genes is more intense (E).
further epigenetic data comparison reveals that although the relevant transcription factors in the ABA response can quickly bind and activate apoptosis gene expression (F), there was no significant change in H3K27me3 modification near these genes (F), thus limiting the increase in apoptosis genes.
, the PRC2 core catalytic enzyme CLF and SWN redundantly mediated H3K27me3 modification and ABA-related transcription factors jointly regulate the expression of ABA-induced aging gene, so that plants respond quickly and moderately to adversity stress in order to better adapt to the environment (G).
Ph.D. students Liu Chunmei and Cheng Jingfei are co-first authors, and the experimental materials and experimental platform have been helped by Xu Lin, a researcher at the Institute of Implant ecology.
related work has been funded by the NSFC project.
Source: Shanghai Institute of Life Sciences.