The preparation of experimental biological reagents is commonly used in laboratories.

related topics .The preparation of
reagents
commonly used in the laboratory: no Ca2 plus, Mg2 plus Hanks liquid, Hanks liquid (original liquid), methyl red reagent, 0.4% phenolic red solution, pH7.2 Tris-NH4CI solution (red cell disintegration solution), 0.05moI/L pH8.6 barbie buffer solution, PBS buffer ), 0.1mol/L PH8.4 boric acid buffer (BBS), 0.05mol/L pH9.6 carbonate buffer, 0.01mol/L pH7.4 PBS containing 2% BSA (cow
serum
albumin), substrate solution, cleaning solution preparation, Cochlear reagent (base test).. 1. No Ca2 plus, Mg2 plus Hanks liquid NaCl 4.5g KCl 0.2g NaHCO3 0.175g Na2HPO4.12H2O 0.076gKH2PO4 0.03g glucose 0.5g 0.4% phenol red 2.5mldissolves or adds the above ingredients to the double steamed water in turn, Finally add double steamed water to 500ml, Adjust the pH to 7.4, 4 degrees C refrigerator to save the spare at 5.6% NaHCO3.. 2. Hanks liquid (original)original liquid A: NaCl 160g KCl 8g MgSO4.7H2O 2g MgCl2.6H2O 2gThe above reagents are added to 800ml of double steamed water. Dissolved..CaCl2 2.8g dissolved in 100ml of double steamed waterthe above two-liquid mixture, plus double steamed water to 1000ml, plus 2 ml of chloroform anti-corrosion, 4 oC preservation.liquid B: Na2NPO4.12H2O 3.04g KH2PO4 1.2g glucose 20g800ml double steamed water, dissolved.0.4% phenolic red solution 100mlthe above two-liquid mixture, plus double steamed water to 1000ml, plus 2 ml chloroform anti-corrosion, 4 degrees C preservation.when using A, B raw liquid according to the following proportions as Hanks liquid use liquid: raw liquid A 1 part, original liquid B 1 part, double steamed water 18 parts filtered, 8 pounds 20min sterilization, put 4 degrees C refrigerator storage, before use with NaHCO3PH adjustment value.. 3. Methyl Red Reagent (1) PartMethyl Red 0.1g Distilled Water 200ml 95% Ethanol 300ml (2) Use: Determination of Methyl Red Reaction.4.0.4% phenolic red solutionsaid to take 0.4g phenol red research, gradually add 0.lmol/LNaOH and continue to grind until all particles are almost completely dissolved, add 0.lmol/LNaOHl0ml, then pour into
capacity bottle
, and add distilled water to l00ml, brown bottle to save spare.. 5.pH7.2 Tris-NH4CI solution (red blood cell disintegration solution) Tris (trihydroxymethane) 1.03gNH4Cl (ammonia chloride) 3.735gplus double steamed water to 500ml, stored at a thick HCl pH of 7.2, 10 lbs 15min after sterilization.6.0.05moI/L pH8.6 barbito bufferbarbito 1.84gplus distilled water 200ml

dissolvedbarbitona 10.3gstacked nitrogen 0.2gplus distilled water dissolved and added to 1000ml.. 7. Phosphate buffer (PBS) A solution: 0.2mol/L sodium dihydrophosphate solution, NaH2PO4 H2O 27.6g, dissolved in distilled water, finally add distilled water to 1000ml..B liquid: 0.2mol/L di sodium phosphate solution, Na2HPO4.7H2O 3.6g (or Na2HPO4.12H2O 71.6g, or Na2HPO4 2H2O 35.6g), dissolved with distilled water, and finally added water to 1000ml. Add distilled water to 200ml to become 0.1mol/LPB.8.0.1mol/L PH8.4 boric acid buffer (BBS) sodium borate (Na2B4O7.10H2O) 0.46g boric acid (H2BO3) 0.51g plus distilled water to 100ml dissolved. 9.0.01mol/L PH7.4 phosphate buffer (PBS) preparation method I: 0.2mol/L Na2HPO4 (Na2HPO4.12H2O 71.6g plus distilled water to 1000ml) 0.2mol/LH2PO4 (NaH2) PO4.2H2O 35.6g plus distilled water to 1000ml) Take 0.2mol/L Na2HPO4 81.0ml plus 0.2mol/L NaH2PO4 19ml, plus 1900ml H2O and 17g NaCl1mol/L PBS. . PH7.4 0.01mol/L PBS adds 0.05% Tween-20 as the washing-up fluid for the ELISA test. : solution (0.1mol/LKH2PO4 solution): KH2PO413.608g, plus distilled water to 1000ml. (0.1mol/L Na2HPO4 solution): Na2HPO435.814g, plus distilled water to 1000ml. 19ml, B 81ml NaCl8.5g, Tween-20 0.5ml mixed with distilled water to 1000ml. Add 0.05% Tween-20 as the washing liquid for the ELISA test. 10.0.05mol/L pH9.6 carbonate buffer Na2CO3 1.59g NaHCO3 2.93g plus distilled water to 1000ml. The ELISA lab bag is used. 11.0.01mol/L pH7.4 PBS contains 2% BSA (bovine serum albumin) take BSA2ml plus 0.01mol/L pH7.4PBS to 100ml. ELISA experiment closed. 12.PH5.0 Phosphoric acid-citric acid buffer 0.2mol/L Na2PO4 (28.4g/L) 25.7ml 0. 1mol/L citric acid (19.2g/L) 24.5ml plus distilled water to 100ml pH 5.0 phosphoric acid-citric acid buffer. 13. Substrate solution pH5.0 phosphoric acid-citric acid buffer added phthalates 1 mg/ml, and then added 30% H2O2 (1 μl/ml) dissolved as ELISA substrate solution, pre-use preparation, avoid-light preservation. 14.2mol/L sulphuric acid H2SO4 (36N) to take 1 ml36N H2SO4 slowly added 18 ml distilled water is 2mol/L, for ELISA experimental termination reaction. 15. The preparation of liquid is high, medium and low. low concentration cleaning liquid potassium heavy chromate 100g water 750ml sulphuric acid 250ml concentration cleaning liquid potassium heavy chromate 60g water 300ml sulphuric acid 4 60ml high concentration cleaning liquid potassium heavy chromate 100g water 200ml sulphuric acid 800ml first pour potassium heavy chromate into the tap water, then add thick sulphuric acid, while adding sulfuric acid and stirring with a glass rod. Due to the addition of thick sulfuric acid to produce high heat, so acid is slow to add, the solution application acid resistance to high temperature plastic or pottery products. clean liquid, which should be stored in a covered glass solution. Glassware that needs to be soaked must be
dying
, if the cleaning liquid has been black for long-term use, indicating that it has failed, it is not suitable for re-use. Because the cleaning fluid is highly corrosive, great care should be taken when operating. 16. Cochlein reagents (indigo-based reagents) (1) parts pure ethyl alcohol 150 ml strong hydrochloric acid 50 ml to dimethylamine formaldehyde 10g (2) method: dimethylamine formaldehyde will be added to pure ethyl alcohol, so that it dissolves. Add a drop of hydrochloric acid slowly, side by side shake, can not be added too fast, so that the temperature rises the solution color darker. (3) use: to determine whether bacteria can produce radon. 17.PH6.4 l/l5mol/ml phosphate buffer salt water (PBS) (1)l/l5mol/ml potassium dihydrophosphate solution KH2PO4 9 .04g distilled water added to 1000ml (2) l/l5mol/ml di sodium phosphate solution Na2HPO4.2H2 O 11.1 87g (or Na2HPO4.l2H2 O 23.86g distilled water plus 1000ml (3) PH6.4 l/l5mol/ml Phosphate buffer salt water (PBS) l/l5mol/ml potassium dihydrophosphate solution 73 ml l/l5mol/ml hydrophosphate di sodium solution 27 ml
.
NaCl 0.5g . Dissolve well. phosphate buffer can be used in cell
culture
, ELISA testing,
immunogroupization
,
organic
solvents and other experiments, mainly to prevent pH changes and solvents of the solution, phosphate buffer reagents mainly composed of sodium chloride, phosphate, phosphate, potassium chloride and potassium phosphate.
.