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The preparation of LB cultures

 Last Update: 2021-01-20
 Source: Internet
 Author: User

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LB< a href" > culturebase, isthe most commonly usedin the "> > microbiology" experiment, is used to culture bacteria such as E. coli, which are classified as liquid or added to a solid-state media made of > agar LB < added to > a href" antibiotic can be used to screen clones hosted by E. coli.

although the name of the media is widely interpreted as the Luria-Bertani media, according to its inventor, Giuseppe Bertani, the name comes from the English word for lysogeny broth, the soluble broth.

LB culture base preparation:

ingredients

tryptone

yeast extract

sodium chloride (NaCl)

Solid media plus agar powder 15-20g

plus double steamed water to 1000mL, pH to 7.2,121C sterilization 30min

preparation method

(3) pH with 1mol/L NaOH solution to 7.2.

(5) add agar to add the required amount of agar,heatingmelt to fill the water loss.

(6) sub-pack, garned, wrapped.

(7) autobaric steam sterilization 100Pa sterilization 20min.

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