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: To inhibit the enzyme action of
protease
on the extracellular substation, I would like to ask, between PH7-8 protease activity can be significantly inhibited? Because PMSF is so toxic, is there any other reagent that inhibits
activity of
enzymes?
EDTA
inhibit metal proteases? Is it related to EDTA chelating Mg, Ca?: Many cytoproteinases only have maximum activity in the acidic environment of lysosomes, pH modulation neutrality will help to minimize protease activity, but still can not completely eliminate its effects, the most common method is to add
protease inhibitors
to control protease hydrolyzing, while operating at 4 degrees as far as possible, PMSF toxicity is indeed very large, it is the inhibitor of serine protease, but generally protein enzyme inhibitors are toxic.
EDTA can chelate the two-price metal cations and inhibit a variety of metal proteases. Giving a list of various protease inhibitors may help you choose the most suitable protease inhibitors..