The researchers discovered key codon repeats that regulate cold tolerance in rice

Crops need to have excellent tolerance modules to cope with abnormal temperatures caused by global climate change, and variety design needs to rely on the principle
of cell cold sensing defense "signal network" and "repair mechanism".
 

In previous research, the team has discovered a series of elements and network relationships between receptors, kinases, chloroplast vitamin E-K1 metabolic pathways, transcription factors and trehalose metabolism in the "signal network" of rice cold perception and defense
。 However, little is known about how the cold-specific DNA repair system is built
.
In their latest study, researchers have found that naturally mutated hardiness gene modules with specific domestication selection can repair DNA damage caused by cold damage
.
Based on the concept of data spatial dimensionality reduction, the genome-wide association analysis of multi-dimensional scale data and dimensionality reduction was carried out by mathematical algorithms, that is, data integration GWAS (DM-GGAS), and the genetic loci and main genes of hardy QTL were systematically identified in rice COLD11, its mutation caused a significant decrease in cold tolerance, the presence of GCG codon duplication in the coding region, and positively correlated with DNA repair activity and cold tolerance, subject to strong domestication selection
.
This is the first time that a novel mechanism
of excellent allele for cold DNA repair by domestication selection has been reported.
This module has important application potential, opening up a new way
for fine regulation of key sites in hardy molecular design breeding.
 

The research results were published online in the international academic journal Science Advances on January 7, and were jointly completed by the Seed Health Research Group of the Institute of Botany, Chinese Academy of Sciences, Li Qizhai, researcher of the Institute of Mathematics and Systems Science, researcher Cheng Zhukuan of the Institute of Genetic Development, and Ge Song, researcher of the Institute of Botany
。 This research was supported
by the Advanced Science and Technology Project of the Chinese Academy of Sciences and the Basic Science Center Project of the National Natural Science Foundation of China.
 

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Domestically selected COLD11 module to repair cold DNA double-strand break pattern diagram

The codon GCG repeat in the first exon of COLD11 was strongly domesticated selected
.
Multi-repeated, highly active modules can respond immediately to DSBs and enable rapid repair when DNA double-strand breaks (DSBs) are induced by low temperature stress, thereby enhancing the cold tolerance
of rice.