The role of cytochrome P450 enzyme in biosynthesis of natural products.

Cytochrome P450 enzymes are a class of ferroglobin-thiol salt (heme-thiolate) protein super-families, best peaks at 450nm after their reduced state is combined with carbon monoxide.
P450 enzyme is widely found in plants and animals and microorganisms, with the characteristics of substrate diversity and catalytic reaction type diversity, in the natural product biosynthesis process plays an important role, known as "multi-talented dancers."
recent years, Li Shengying's team at Qingdao Institute of Bioeneding and Process Research of the Chinese Academy of Sciences has carried out a series of studies on the biochemical function, catalytic mechanism and protein structure of P450 enzymes, and the results were published in Nat.Chem. (2011), J.Am.Chem.Soc. (2011, 2014), Proc.Natl.Acad.Sci.U.S.A. (2009, 2017), Chem.Biol. (2008), Biotechnol.Biofuels (2014, 2015), Sci.Rep. (2017), J.Biol.Chem. (2006, 2009, 2012, 2016), Appl.Environ.Microbiol (2015), etc., has attracted the attention of domestic and foreign counterparts.
recently, the results of the study, published in Nature Product Reports under the title Ofansion of chemical space for natural products by uncommon P450 reactions, review and look forward to the above results and the "unconventional" P450 response in recent literature reports.
the paper explains the important role of natural product biosynthesis and P450 enzyme in this process, summarizes the classical P450 catalytic process, and briefly describes the "conventional" reaction types (hydroxylation, cycloridation reactions) of P450 catalysis in the process of natural product biosynthesis. The
article provides a detailed review of the "unconventional" P450 reaction, which categorizes, summarizes the role of the "unconventional" P450 reaction in expanding the natural product skeleton and structural diversity by citing classic examples of "new chemistry", "skeleton construction" and "structural re-shaping" associated with P450 enzyme reactions found in recent years.
li Shengying's team found that P450 enzymes can produce new reactive activity by interacting with third-party proteins from other non-reduced partners, and this paper is the first systematic review of these new findings.
paper summarizes the catalytic logic of "unconventional" P450 enzyme in the biosynthesis process of natural products, and looks forward to the future research direction and prospects of "unconventional" P450 reaction.
the cover image of the 9th issue of Natural Product Reports 2017, Li Shengying's team compared the changing role of P450 enzymes in expanding the structural diversity of natural products by using the cartoon image of Sun Wukong and his "72 Changes" skills.
the use and modification of the cartoon image of "Sun Wukong" has been officially authorized by Shanghai Fine Arts Film Studio.
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