The role of H3K27me3 modification and DNA methylation in the fate of embryos.

Recently, the Institute of Biochemistry and Cell Biology of the Shanghai Institute of Life Sciences of the Chinese Academy of Sciences, Jing Nai-chi Research Group, in collaboration with the Tang Fuyu Research Group of Peking University, with silencing of development al-genes by H3K27me3 and DNA research reflects the sprepant plastic ity of the company and the embryonic soping line line, published online in Cell Research.
the study revealed the changes of DNA methylation and H3K27me3 modification patterns of cells in different parts of the embryo during the primary intestinal movement period, and found the phenomenon of development-related genes enriching DNA methylation modification in the exomer region and H3K27me3 modification in the embryo region, and clarified that the two episomentomic regulatory mechanisms play an important role in the differences in the potential of the development of cells in the embryo and embryo parts. The regulatory mechanism of
the fate of cells is one of the key problems in the field of developmental biology and stem cell research.
during the early development of mouse embryos, omnipotent heron cells are gradually specialized in the formation of nourishing outer embryo layer (TE) and intracellular group (ICM) cells after continuous cell proliferation.
te then forms cells such as the outer embryo layer, and eventually the placental tissue, etc., while ICM cells develop and form the original endosperm cell and the upper embryo layer cell, the upper embryo layer cell forms the outer, medium and inner three germ layers through the original intestine movement, and will develop into a complete individual in the future.
epigenetic regulation plays an important role in this process.
the researchers, by establishing chromatin immunoprecipitation technology based on a small number of cells and sulphite sequencing technology, studied the changes and distribution characteristics of histone H3K27me3 modification and DNA methylation modification of cells in different parts of the embryo after bed in mice, and found and verified the differences in the regulatory mechanisms of the development-related genes in the embryo region and the outer embryo region and their impact on the development potential.
the results of this study provide a new perspective and theoretical guidance for clarifying the effects of different epigenetic regulations on cell destiny and developmental plasticity, understanding the omnipotent and differentiation potential of stem cells, and the application of stem cell translational medicine.
research work has been supported by the Chinese Academy of Sciences' strategic leading science and technology project, the Ministry of Science and Technology and the National Natural Science Foundation of China. the data collection of
has been supported by the chemical and biological laboratory animal experimental technology platform, the molecular biology technology platform and the cell analysis technology platform.
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