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Heat shock proteins (HSPs) are rapidly induced after stresses, such as heat shock, and accumulate at high concentrations in cells. HSP induction involves a family of heat shock transcription factors that bind the heat shock elements of the
HSP
genes and mediate transcription
in trans
. We discuss methods for the study of HSP binding to
HSP
promoters and the consequent increases in HSP gene expression in vitro and in vivo.