The triamcinolone metabolic pathway regulates the formation of pollen packets and the infertility of wet-sensitive males in rice.

Pollen grains in higher plants have a hard outer wall of pollen that contains a substance called "powder pack".
pollen pack seislus to prevent hay grain drying, protect pollen grains from mechanical damage and pathogen infection.
in nature, the plants with poor growth of pollen walls and lack of pollen packets showed a marked decline in fertility.
study the formation mechanism of pollen packs is of great significance for the discovery of good male infertility materials needed for crop hybrid breeding and breeding.
researcher of the Institute of Plant Research of the Chinese Academy of Sciences, the study group of Yu Koizumi has been engaged in the study of plant metabolism for a long time, and in the early studies has identified several plant diazenota and triamcinolone metabolic pathways.
recently, the researchers of the research team found that rice triamcinase OsOSC12/OsPTS1 can catalyze 2,3-epoxy sachoene to produce a dicyclic triamcinoline compound "yuguvelvet felt alcohol";
this mutant is sterile in an environment with humidity below 60%, while in an environment with humidity above 80%, it is fully fertile, and it is a new type of humidity-sensitive male nuclear infertility material. Further studies
showed that the pollen pack of mutants was significantly reduced in palmic acid and linolenic acid than normal plants, and was completely short of steaic acid, and that the addition of palmic acid and linoleic acid to the mutant's pollen grains was effective in preventing excessive loss of water in the pollen grains, thus showing that the lack of these three fatty acids was the main cause of rapid loss of pollen grains.
taking into account the prevalence of OsOSC12/OsPTS1 in the plants, it has been found that the gene is expressed in corn, wheat and barley flower medicine, and the detection of the presence of "granud in the velvet felt alcohol", indicating the existence of similar molecular mechanisms in the plant.
triamcinolone metabolic pathway regulates the formation of pollen packets and rice wet-sensitive male infertility.
A, OsOSC12/OsPTS1)-guided metabolic pathways regulate the pattern of fatty acid accumulation in the outer walls of pollen (flower drug development period 9-12); B, wet-sensitive male sterile mutants (E157) in a dry and highly humid environment of strong rice spikes.
this study reveals the key role of OsOSC12/OsPTS1 in the formation of rice pollen packs, which provides a basis for an in-depth understanding of the formation mechanism and water retention mechanism of pollen packs.
, the study also obtained rice humidity-sensitive male infertility, which is another conditional male infertility material after the early discovery of rice light/temperature-sensitive male infertility material.
due to the early detection of light /temperature-sensitive male infertility system of breeding needs to strictly control the temperature or light, greatly limit its application range, and the new sterile system of breeding only by the influence of the ambient humidity, can be in the summer drought and rain less areas to prepare hybrids, so as to break through the current rice hybrid advantage utilization of the geographical restrictions.
researchers believe that using the same research methods and techniques, it is hoped that a variety of wet-sensitive male infertility materials from undergraduate crops will be obtained, opening a new chapter in the hybrid breeding of two-line crops and the utilization of hybrid advantages.
related research results published in Nature-Communication, Yu Koizumi Research Group Associate Researcher Xue Zheyong, Ph.D. graduate Xu Xia, Ph.D. graduate Zhou Wei as co-first author, Yu Koizumi as the author of communications.
the research was funded by the National Natural Science Foundation of China's key projects and the National Key Basic Research and Development Program.
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