The world's first robot-operated sotic cell cloned pig was born in Tianjin, China.

Recently, after more than two months of long waiting, a special "parent-child identification" report came out, 13 cloned piglets and "surrogate" mother has no blood relationship, only with the supply cells there is a "parent-child relationship."
this medically proven that the world's first robot-operated sotic cell cloned pig was born in Tianjin, China.
after 110 days of conceived, on April 26th and 29th, two ordinary "surrogate" sows successfully gave birth to 13 healthy purebred white pigs.
Compared with the previous "manual operation" cloning technology, the robot's automated "knife", with less force, less damage to cells, more accurate operation will be the key indicator of the success of sotic cell cloning technology "blast rate" from 10% to 20%.
the figure surprised peers around the world.
"Robot Operating Body Cell Clone Pig" research is an interdisciplinary research team led by Professor Zhao Xin of Nanke University Robotics Institute, with Tianjin Institute of Livestock and Veterinary Medicine as the main partner.
"Micro-operated Robotic System for Biomedical Engineering" developed by Nanke University Robotics for many years, won the second prize of national technology invention in 2002.
the birth of the cloned pig, but also 15 years later, the technology made another major breakthrough.
cell cloning is one of the classical methods to improve biological varieties.
it injects the nucleums of common varieties of ovaries into the cells of good varieties.
advantage of this approach is that the obtained offspring must be good varieties.
however, the success rate is very low, which becomes the bottleneck of the development of sotic cell cloning technology.
as one of the most complex microoperations, soy cell cloning technology urgently needs to be roboticized.
is a real challenge for us to improve the success rate of subsequent cell culture through the operation of robotic cell nuclear transplantation, which in turn can improve the success rate of whole sotic cell cloning technology.
," Zhao xin said.
In response to the above-mentioned problems, Nanke University research team for the development of life science needs to carry out scientific research, developed with visualization, minimally invastured, fixed-point, quantitative function, set detection analysis and operation in one of the in-place microanalysis and operating instrument, the use of this instrument to achieve a robotic cell nuclear transplantation process.
, the key difficulty of soy cell cloning technology based on robot microoperation is how to minimize damage to cells.
team at Nanke University in The New York City, China, designed and implemented a robotic cell nuclear transplantation process that included ovary trots to find the polar body, pumping nuclei, and somatic cell injection.
by analyzing the cell receptive conditions in the contact between micro-operating tools and cells, the researchers realized the cell twitching and pumping nucleus based on the minimum force, respectively, and ensured that the cells had the least force during the operation of cell nuclear transplantation.
" manual operation of the tying cells, cells up to 30 to 40 microns deformation, calculated robot operating cells maximum deformation reduced to 10 to 15 microns.
if the strength of the past is 'a heavy punch', then now is 'a single push'.
" Zhao Xin introduced, the micro operating system based on the balanced pressure model operation, but also make the process of extracting the nucleuter more "controllable" and "gentle."
results show that cell twitching method based on minimum force significantly reduces cell damage, and subsequent cell culture shows that cell follow-up development rate is significantly higher than that of cell pumping operation based on minimum force compared to manual operation.
most exciting is that in in-body experiments we used this micro-operating platform to perform nuclear transplants on 53 ovaries, 11 of which developed into blastocysts that marked successful cloning, with a development rate of 21%.
the previous body cell cloning blastocyst rate has been maintained at about 10%.
," Zhao xin said.
in order for the technology to "blossom", Nan kai University's research team applied the nuclear transplant method to the entire cloning pig process, and completed thousands of nuclear transplants to verify the effectiveness of the method.
January 2017, 510 nuclear transplants were completed in four batches and 510 cloned embryos were transplanted into six sows.
, the two sows successfully conceived and produced seven and six pigs on April 26 and 29, respectively, for a total of 13 healthy cloned pigs.
"Our study is the first to guide microoperation from the perspective of cell development, linking microoperation processes to cell development results through cell stress.
this method can further improve the contribution of micro-operation technology to the overall biological process.
predictably, it has good application prospects in the fields of assisted fertility, animal and plant species improvement, and mass medicine and livestock production.
," Zhao xin said.
, it is understood that the project has been the national major scientific research equipment development special, "863" plan advanced manufacturing field, Tianjin intelligent robot major science and technology special support.
(Zhang Daozheng Wu Junhui)